PMID- 10570147
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 96
IP  - 24
DP  - 1999 Nov 23
TI  - Genetic dissection of dome formation in a mammary cell line: identification of
      two genes with opposing action.
PG  - 13766-70
AB  - In this work, we extend the study of the genes controlling the formation of domes
      in the rat mammary cell line LA7 under the influence of DMSO. The role of the
      rat8 gene has already been demonstrated. We have now studied two additional
      genes. The first, called 133, is the rat ortholog of the human epithelial
      membrane protein 3 (EMP3), a member of the peripheral myelin protein 22
      (PMP22)/EMP/lens-specific membrane protein 20 (MP20) gene family that encodes for
      tetratransmembrane proteins; it is expressed in the LA7 line in the absence of
      DMSO but not in its presence. The second gene is the beta subunit of the
      amiloride-sensitive Na(+) channel. Studies with antisense oligonucleotides show
      that the formation of domes is under the control of all three genes: the
      expression of rat8 is required for both their formation and their persistence;
      the expression of the Na(+) channel beta subunit is required for their formation;
      and the expression of gene 133 blocks the expression of the Na(+) channel genes, 
      thus preventing formation of the domes. The formation of these structures is also
      accompanied by the expression of alpha(6)beta(1) integrin, followed by that of
      E-cadherin and cytokeratin 8. It appears, therefore, that dome formation requires
      the activity of the Na(+) channel and the rat8-encoded protein and is under the
      negative control of gene 133. DMSO induces dome formation by blocking this
      control.
FAU - Zucchi, I
AU  - Zucchi I
AD  - Istituto di Tecnologie Biomediche Avanzate, Consiglio Nazionale delle Ricerche,
      Via Fratelli Cervi 93, 20090 Segrate, Milan, Italy. zucchi@itba.mi.cnr.it
FAU - Montagna, C
AU  - Montagna C
FAU - Susani, L
AU  - Susani L
FAU - Montesano, R
AU  - Montesano R
FAU - Affer, M
AU  - Affer M
FAU - Zanotti, S
AU  - Zanotti S
FAU - Redolfi, E
AU  - Redolfi E
FAU - Vezzoni, P
AU  - Vezzoni P
FAU - Dulbecco, R
AU  - Dulbecco R
LA  - eng
SI  - GENBANK/Y10889
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Biomarkers)
RN  - 0 (Cadherins)
RN  - 0 (EMP3 protein, human)
RN  - 0 (Emp3 protein, rat)
RN  - 0 (Epithelial Sodium Channels)
RN  - 0 (Integrin alpha6beta1)
RN  - 0 (Integrins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Sodium Channels)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Biomarkers
MH  - Cadherins/genetics
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Epithelial Sodium Channels
MH  - Gene Expression Regulation
MH  - Humans
MH  - Integrin alpha6beta1
MH  - Integrins/biosynthesis
MH  - Keratins/genetics
MH  - Mammals
MH  - Membrane Glycoproteins/*genetics
MH  - Molecular Sequence Data
MH  - Rats
MH  - Sodium Channels/*genetics
PMC - PMC24139
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1073/pnas.96.24.13766 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13766-70. doi:
      10.1073/pnas.96.24.13766.