PMID- 10570015
OWN - NLM
STAT- MEDLINE
DCOM- 19991221
LR  - 20190513
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 61
IP  - 6
DP  - 1999 Dec
TI  - Expression of recombinant human zona pellucida protein 2 and its binding capacity
      to spermatozoa.
PG  - 1649-54
AB  - The human zona pellucida (ZP) is composed of three major glycoproteins: ZP1, ZP2,
      and ZP3. The aim of this study was to clarify the role of ZP2 by focusing on the 
      polypeptide structure. We produced in Escherichia coli a recombinant human ZP2
      protein (rec-hZP2) corresponding to amino acid sequence 1-206 of the mature
      protein. The final yield of rec-hZP2 protein was 80 microg/ml Luria Broth medium.
      After 2-h incubation of human spermatozoa with rec-hZP2 in vitro, an
      immunofluorescent study indicated that rec-hZP2 bound only to acrosome-reacted
      spermatozoa. The binding site migrated from the acrosome to the midpiece of the
      spermatozoa. Rabbit and mouse antisera produced against rec-hZP2 stained native
      human ZP in the immunofluorescent study, and significantly blocked human sperm
      binding and penetration into human ZP as compared to control values. The
      N-terminal polypeptide portion of human ZP2 was shown to contain a binding site
      for acrosome-reacted spermatozoa and to play an important role in secondary sperm
      binding and penetration into the ZP.
FAU - Tsubamoto, H
AU  - Tsubamoto H
AD  - Department of Obstetrics and Gynecology, Institute of Advanced Medical Sciences, 
      Hyogo College of Medicine, Nishinomiya, 663-8501, Japan.
FAU - Hasegawa, A
AU  - Hasegawa A
FAU - Nakata, Y
AU  - Nakata Y
FAU - Naito, S
AU  - Naito S
FAU - Yamasaki, N
AU  - Yamasaki N
FAU - Koyama, K
AU  - Koyama K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Egg Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Recombinant Proteins)
RN  - 0 (ZP1 protein, human)
RN  - 0 (ZP2 protein, human)
RN  - 0 (ZP3 protein, human)
RN  - 0 (Zona Pellucida Glycoproteins)
RN  - 0 (Zp1 protein, mouse)
RN  - 0 (Zp2 protein, mouse)
RN  - 0 (Zp3 protein, mouse)
SB  - IM
MH  - Acrosome/metabolism
MH  - Acrosome Reaction
MH  - Animals
MH  - Binding Sites
MH  - Egg Proteins/*genetics/*metabolism
MH  - Escherichia coli/genetics
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - *Gene Expression
MH  - Humans
MH  - Male
MH  - Membrane Glycoproteins/*genetics/*metabolism
MH  - Mice
MH  - Rabbits
MH  - *Receptors, Cell Surface
MH  - Recombinant Proteins/metabolism
MH  - Sperm-Ovum Interactions
MH  - Spermatozoa/*metabolism
MH  - Zona Pellucida Glycoproteins
EDAT- 1999/11/26 00:00
MHDA- 1999/11/26 00:01
CRDT- 1999/11/26 00:00
PHST- 1999/11/26 00:00 [pubmed]
PHST- 1999/11/26 00:01 [medline]
PHST- 1999/11/26 00:00 [entrez]
AID - 10.1095/biolreprod61.6.1649 [doi]
PST - ppublish
SO  - Biol Reprod. 1999 Dec;61(6):1649-54. doi: 10.1095/biolreprod61.6.1649.