PMID- 10567720
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 461
IP  - 3
DP  - 1999 Nov 19
TI  - Kinetic mechanism and ATP-binding site reactivity of p38gamma MAP kinase.
PG  - 323-8
AB  - Activated p38gamma MAP kinase exhibited significant basal ATPase activity in the 
      absence of a kinase substrate, and addition of a phosphoacceptor substrate
      increased k(cat)/K(m)20-fold. AMP-PCP was competitive with ATP binding and
      non-competitive with phosphoacceptor substrate binding. The nucleotide binding
      site affinity label 5'-(p-fluorosulfonylbenzoyl)adenosine (FSBA) bound
      stoichiometrically at Lys-56 in the ATP site of both unphosphorylated and
      activated p38gamma. AMP-PCP only protected the activated enzyme from FSBA
      inactivation, implying that AMP-PCP does not bind unphosphorylated p38gamma.
      Basal ATPase activities were also observed for activated p38alpha, ERK2 and JNK3 
      suggesting that the enzymatic mechanism may be similar for all classes of MAP
      kinases.
FAU - Fox, T
AU  - Fox T
AD  - Vertex Pharmaceuticals Incorporated, 130 Waverly Street, Cambridge, MA
      02139-4242, USA. Fox@vpharm.com
FAU - Fitzgibbon, M J
AU  - Fitzgibbon MJ
FAU - Fleming, M A
AU  - Fleming MA
FAU - Hsiao, H M
AU  - Hsiao HM
FAU - Brummel, C L
AU  - Brummel CL
FAU - Su, M S
AU  - Su MS
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Affinity Labels)
RN  - 3469-78-1 (5'-adenylyl (beta,gamma-methylene)diphosphonate)
RN  - 78859-42-4 (5'-(4-fluorosulfonylbenzoyl)adenosine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.- (Mitogen-Activated Protein Kinase 10)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - K72T3FS567 (Adenosine)
RN  - NYX13NT29D (alpha,beta-methyleneadenosine 5'-triphosphate)
SB  - IM
MH  - Adenosine/analogs & derivatives/metabolism
MH  - Adenosine Triphosphatases/chemistry/*metabolism
MH  - Adenosine Triphosphate/analogs & derivatives/*metabolism
MH  - Affinity Labels
MH  - Allosteric Site
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - Binding, Competitive
MH  - Enzyme Activation
MH  - Kinetics
MH  - MAP Kinase Signaling System
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 10
MH  - Mitogen-Activated Protein Kinases/chemistry/*metabolism
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Processing, Post-Translational
MH  - Protein-Tyrosine Kinases/metabolism
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0014-5793(99)01488-X [pii]
AID - 10.1016/s0014-5793(99)01488-x [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Nov 19;461(3):323-8. doi: 10.1016/s0014-5793(99)01488-x.