PMID- 10567706
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20191210
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 461
IP  - 3
DP  - 1999 Nov 19
TI  - Interactions between the full complement of human RNA polymerase II subunits.
PG  - 253-7
AB  - As an approach to elucidating the rules governing the assembly of human RNA
      polymerase II (hRPB), interactions between its subunits have been systematically 
      analyzed. Eleven of the 12 expected hRPB subunits have previously been tested for
      reciprocal interactions (J. Biol. Chem. 272 (1997) 16815-16821). We now report
      the results obtained for the last subunit (hRPB4; Mol. Cell. Biol. 18 (1998)
      1935-1945) and propose an essentially complete picture of the potential
      interactions occurring within hRPB. Finally, complementation experiments in yeast
      indicated that hRPB4 expression efficiently cured both heat and cold-sensitivity 
      of RPB4-lacking strains, supporting the existence of conserved functional subunit
      interactions.
FAU - Schaller, S
AU  - Schaller S
AD  - Institut de Genetique et de Biologie Moleculaire et Cellulaire (CNRS/INSERM/ULP),
      BP 163, 67404, Illkirch, France.
FAU - Grandemange, S
AU  - Grandemange S
FAU - Shpakovski, G V
AU  - Shpakovski GV
FAU - Golemis, E A
AU  - Golemis EA
FAU - Kedinger, C
AU  - Kedinger C
FAU - Vigneron, M
AU  - Vigneron M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA, Complementary/genetics
MH  - Genetic Complementation Test
MH  - Humans
MH  - Nucleopolyhedroviruses/genetics
MH  - Protein Conformation
MH  - RNA Polymerase II/*chemistry/physiology
MH  - Recombinant Fusion Proteins/physiology
MH  - Saccharomyces cerevisiae/genetics/physiology
MH  - Spodoptera
MH  - Temperature
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0014-5793(99)01441-6 [pii]
AID - 10.1016/s0014-5793(99)01441-6 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Nov 19;461(3):253-7. doi: 10.1016/s0014-5793(99)01441-6.