PMID- 10567642
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20200217
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 80 ( Pt 12)
DP  - 1999 Dec
TI  - Characterization of infectious Murray Valley encephalitis virus derived from a
      stably cloned genome-length cDNA.
PG  - 3115-3125
LID - 10.1099/0022-1317-80-12-3115 [doi]
AB  - An infectious cDNA clone of Murray Valley encephalitis virus prototype strain
      1-51 (MVE-1-51) was constructed by stably inserting genome-length cDNA into the
      low-copy-number plasmid vector pMC18. Designated pMVE-1-51, the clone consisted
      of genome-length cDNA of MVE-1-51 under the control of a T7 RNA polymerase
      promoter. The clone was constructed by using existing components of a cDNA
      library, in addition to cDNA of the 3' terminus derived by RT-PCR of
      poly(A)-tailed viral RNA. Upon comparison with other flavivirus sequences, the
      previously undetermined sequence of the 3' UTR was found to contain elements
      conserved throughout the genus FLAVIVIRUS: RNA transcribed from pMVE-1-51 and
      subsequently transfected into BHK-21 cells generated infectious virus. The plaque
      morphology, replication kinetics and antigenic profile of clone-derived virus
      (CDV-1-51) was similar to the parental virus in vitro. Furthermore, the virulence
      properties of CDV-1-51 and MVE-1-51 (LD(50) values and mortality profiles) were
      found to be identical in vivo in the mouse model. Through site-directed
      mutagenesis, the infectious clone should serve as a valuable tool for
      investigating the molecular determinants of virulence in MVE virus.
FAU - Hurrelbrink, Robert J
AU  - Hurrelbrink RJ
AD  - Department of Microbiology, University of Western Australia, Nedlands, WA 6907,
      Australia1.
FAU - Nestorowicz, Ann
AU  - Nestorowicz A
AD  - Endocrine Division, Lilly Research Laboratories, Indianapolis, IN 46285, USA2.
FAU - McMinn, Peter C
AU  - McMinn PC
AD  - Department of Microbiology, University of Western Australia, Nedlands, WA 6907,
      Australia1.
LA  - eng
SI  - GENBANK/AF161266
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (3' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Encephalitis Virus, Murray Valley/*genetics/immunology/*pathogenicity
MH  - Encephalitis, Arbovirus/pathology/*virology
MH  - Fluorescent Antibody Technique
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Precipitin Tests
MH  - RNA, Viral/biosynthesis/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Vero Cells
MH  - Viral Plaque Assay
MH  - Virulence
MH  - Virus Replication
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1099/0022-1317-80-12-3115 [doi]
PST - ppublish
SO  - J Gen Virol. 1999 Dec;80 ( Pt 12):3115-3125. doi: 10.1099/0022-1317-80-12-3115.