PMID- 10567585
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 12
DP  - 1999 Dec
TI  - Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear
      protein export.
PG  - 8616-24
AB  - Active transport of macromolecules between the nucleus and cytoplasm requires
      signals for import and export and their recognition by shuttling receptors. Each 
      class of macromolecule is thought to have a distinct receptor that mediates the
      transport reaction. Assembly and disassembly reactions of receptor-substrate
      complexes are coordinated by Ran, a GTP-binding protein whose nucleotide state is
      regulated catalytically by effector proteins. Ran function is modulated in a
      noncatalytic fashion by NTF2, a protein that mediates nuclear import of Ran-GDP. 
      Here we characterize a novel component of the Ran system that is 26% identical to
      NTF2, which based on its function we refer to as NTF2-related export protein 1
      (NXT1). In contrast to NTF2, NXT1 preferentially binds Ran-GTP, and it
      colocalizes with the nuclear pore complex (NPC) in mammalian cells. These
      properties, together with the fact that NXT1 shuttles between the nucleus and the
      cytoplasm, suggest an active role in nuclear transport. Indeed, NXT1 stimulates
      nuclear protein export of the NES-containing protein PKI in vitro. The export
      function of NXT1 is blocked by the addition of leptomycin B, a compound that
      selectively inhibits the NES receptor Crm1. Thus, NXT1 regulates the
      Crm1-dependent export pathway through its direct interaction with Ran-GTP.
FAU - Black, B E
AU  - Black BE
AD  - Center for Cell Signaling, University of Virginia Health Sciences Center,
      Charlottesville, Virginia 22908, USA.
FAU - Levesque, L
AU  - Levesque L
FAU - Holaska, J M
AU  - Holaska JM
FAU - Wood, T C
AU  - Wood TC
FAU - Paschal, B M
AU  - Paschal BM
LA  - eng
SI  - GENBANK/AF156957
SI  - GENBANK/AF156958
SI  - GENBANK/AF156959
SI  - GENBANK/AF156960
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (NXT1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (Nutf2 protein, mouse)
RN  - 0 (Nxt1 protein, mouse)
RN  - 0 (nxt-1 protein, C elegans)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Biological Transport
MH  - Caenorhabditis elegans
MH  - Caenorhabditis elegans Proteins
MH  - Carrier Proteins/classification/*genetics/*metabolism/physiology
MH  - DNA, Complementary
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Envelope
MH  - Nuclear Proteins/*genetics/*metabolism/physiology
MH  - *Nucleocytoplasmic Transport Proteins
MH  - Sequence Homology, Amino Acid
MH  - ran GTP-Binding Protein/*metabolism
PMC - PMC84993
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1128/mcb.19.12.8616 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Dec;19(12):8616-24. doi: 10.1128/mcb.19.12.8616.