PMID- 10567526
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 12
DP  - 1999 Dec
TI  - Biochemical analysis of the intrinsic Mcm4-Mcm6-mcm7 DNA helicase activity.
PG  - 8003-15
AB  - Mcm proteins play an essential role in eukaryotic DNA replication, but their
      biochemical functions are poorly understood. Recently, we reported that a DNA
      helicase activity is associated with an Mcm4-Mcm6-Mcm7 (Mcm4,6,7) complex,
      suggesting that this complex is involved in the initiation of DNA replication as 
      a DNA-unwinding enzyme. In this study, we have expressed and isolated the mouse
      Mcm2, 4,6,7 proteins from insect cells and characterized various mutant Mcm4,6,7 
      complexes in which the conserved ATPase motifs of the Mcm4 and Mcm6 proteins were
      mutated. The activities associated with such preparations demonstrated that the
      DNA helicase activity is intrinsically associated with the Mcm4,6,7 complex.
      Biochemical analyses of these mutant Mcm4,6,7 complexes indicated that the ATP
      binding activity of the Mcm6 protein in the complex is critical for DNA helicase 
      activity and that the Mcm4 protein may play a role in the single-stranded DNA
      binding activity of the complex. The results also indicated that the two
      activities of DNA helicase and single-stranded DNA binding can be separated.
FAU - You, Z
AU  - You Z
AD  - Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194-8511, Japan.
FAU - Komamura, Y
AU  - Komamura Y
FAU - Ishimi, Y
AU  - Ishimi Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (MCM4 protein, S cerevisiae)
RN  - EC 3.6.4.12 (MCM6 protein, S cerevisiae)
RN  - EC 3.6.4.12 (MCM7 protein, S cerevisiae)
RN  - EC 3.6.4.12 (Mcm6 protein, mouse)
RN  - EC 3.6.4.12 (Mcm7 protein, mouse)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 2)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 4)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 6)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 7)
SB  - IM
MH  - Adenosine Triphosphatases/metabolism
MH  - Animals
MH  - Cell Cycle Proteins/genetics/isolation & purification/*metabolism
MH  - Chromosomal Proteins, Non-Histone
MH  - DNA Helicases/genetics/isolation & purification/*metabolism
MH  - DNA, Single-Stranded/metabolism
MH  - DNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Gene Expression
MH  - Mice
MH  - Minichromosome Maintenance Complex Component 2
MH  - Minichromosome Maintenance Complex Component 4
MH  - Minichromosome Maintenance Complex Component 6
MH  - Minichromosome Maintenance Complex Component 7
MH  - Mutagenesis
MH  - Nuclear Proteins/genetics/isolation & purification/*metabolism
MH  - Protein Structure, Tertiary
MH  - *Saccharomyces cerevisiae Proteins
PMC - PMC84885
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1128/mcb.19.12.8003 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Dec;19(12):8003-15. doi: 10.1128/mcb.19.12.8003.