PMID- 10567518
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20190508
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 19
IP  - 12
DP  - 1999 Dec
TI  - Isolation of an FMRP-associated messenger ribonucleoprotein particle and
      identification of nucleolin and the fragile X-related proteins as components of
      the complex.
PG  - 7925-32
AB  - The loss of FMR1 expression due to trinucleotide repeat expansion leads to
      fragile X syndrome, a cause of mental retardation. The encoded protein, FMRP, is 
      a member of a gene family that also contains the fragile X-related proteins,
      FXR1P and FXR2P. FMRP has been shown to be a nucleocytoplasmic shuttling protein 
      that selectively binds a subset of mRNAs, forms messenger ribonucleoprotein
      (mRNP) complexes, and associates with translating ribosomes. Here we describe a
      cell culture system from which we can isolate epitope-tagged FMRP along with
      mRNA, including its own message, and at least six other proteins. We identify two
      of these proteins as FXR1P and FXR2P by using specific antisera and identify a
      third protein as nucleolin by using mass spectrometry. The presence of nucleolin 
      is confirmed by both reactivity with a specific antiserum as well as reverse
      coimmunoprecipitation where antinucleolin antiserum immunoprecipitates endogenous
      FMRP from both cultured cells and mouse brain. The identification of nucleolin, a
      known component of other mRNPs, adds a new dimension to the analysis of FMRP
      function, and the approach described should also allow the identification of the 
      remaining unknown proteins of this FMRP-associated mRNP as well as the other
      bound mRNAs.
FAU - Ceman, S
AU  - Ceman S
AD  - Howard Hughes Medical Institute and Departments of Biochemistry, Pediatrics, and 
      Genetics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
FAU - Brown, V
AU  - Brown V
FAU - Warren, S T
AU  - Warren ST
LA  - eng
GR  - P01 HD035576/HD/NICHD NIH HHS/United States
GR  - R37 HD020521/HD/NICHD NIH HHS/United States
GR  - P01HD35576/HD/NICHD NIH HHS/United States
GR  - R37HD20521/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (FXR1 protein, human)
RN  - 0 (FXR2 protein, human)
RN  - 0 (Fmr1 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptides)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (nucleolin)
RN  - 139135-51-6 (Fragile X Mental Retardation Protein)
RN  - 98849-88-8 (FLAG peptide)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Fragile X Mental Retardation Protein
MH  - *Fragile X Syndrome
MH  - Gene Expression
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Tissue Proteins/genetics/isolation & purification/*metabolism
MH  - Oligopeptides
MH  - Peptides/genetics
MH  - Phosphoproteins/isolation & purification/*metabolism
MH  - Precipitin Tests
MH  - RNA, Messenger
MH  - RNA-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Recombinant Proteins/genetics/isolation & purification/metabolism
MH  - Ribonucleases/metabolism
MH  - Ribonucleoproteins/genetics/isolation & purification/*metabolism
PMC - PMC84877
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1128/mcb.19.12.7925 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1999 Dec;19(12):7925-32. doi: 10.1128/mcb.19.12.7925.