PMID- 10567410
OWN - NLM
STAT- MEDLINE
DCOM- 19991229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 48
DP  - 1999 Nov 26
TI  - Human 75-kDa DNA-pairing protein is identical to the pro-oncoprotein TLS/FUS and 
      is able to promote D-loop formation.
PG  - 34337-42
AB  - Homologous recombination plays a fundamental role in DNA double-strand break
      repair. Previously, we detected two mammalian nuclear proteins of 100 and 75 kDa 
      (POMp100 and POMp75, respectively) that are able to promote homologous DNA
      pairing, a key step in homologous recombination. Here we describe the
      identification of human (h) POMp75 as the pro-oncoprotein TLS/FUS. hPOMp75/TLS
      binds both single- and double-stranded DNAs and mediates annealing of
      complementary DNA strands. More important, it promotes the uptake of a
      single-stranded oligonucleotide into a homologous superhelical DNA to form a
      D-loop. The formation of a D-loop is an essential step in DNA double-strand break
      repair through recombination. DNA annealing and D-loop formation catalyzed by
      hPOMp75/TLS require Mg(2+) and are ATP-independent. Interestingly, the oncogenic 
      fusion form TLS-CHOP is not able to promote DNA pairing. These data suggest a
      possible role for hPOMp75/TLS in maintenance of genomic integrity.
FAU - Baechtold, H
AU  - Baechtold H
AD  - Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel,
      Switzerland.
FAU - Kuroda, M
AU  - Kuroda M
FAU - Sok, J
AU  - Sok J
FAU - Ron, D
AU  - Ron D
FAU - Lopez, B S
AU  - Lopez BS
FAU - Akhmedov, A T
AU  - Akhmedov AT
LA  - eng
GR  - CA60945/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoproteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (RNA-Binding Protein FUS)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (TLS-CHOP fusion protein, human)
RN  - 147336-12-7 (Transcription Factor CHOP)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Pairing
MH  - Binding, Competitive
MH  - *CCAAT-Enhancer-Binding Proteins
MH  - DNA/chemistry/genetics/*metabolism
MH  - DNA, Single-Stranded/chemistry/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - HeLa Cells
MH  - Heterogeneous-Nuclear Ribonucleoproteins
MH  - Humans
MH  - Molecular Weight
MH  - Nuclear Proteins/chemistry/isolation & purification/*metabolism
MH  - Nucleic Acid Conformation
MH  - Oncogene Proteins, Fusion/metabolism
MH  - RNA-Binding Protein FUS
MH  - Ribonucleoproteins/*metabolism
MH  - Transcription Factor CHOP
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.48.34337 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 26;274(48):34337-42. doi: 10.1074/jbc.274.48.34337.