PMID- 10567404
OWN - NLM
STAT- MEDLINE
DCOM- 19991229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 48
DP  - 1999 Nov 26
TI  - A nuclear factor, ASC-2, as a cancer-amplified transcriptional coactivator
      essential for ligand-dependent transactivation by nuclear receptors in vivo.
PG  - 34283-93
AB  - Many transcription coactivators interact with nuclear receptors in a ligand- and 
      C-terminal transactivation function (AF2)-dependent manner. We isolated a nuclear
      factor (designated ASC-2) with such properties by using the ligand-binding domain
      of retinoid X receptor as a bait in a yeast two-hybrid screening. ASC-2 also
      interacted with other nuclear receptors, including retinoic acid receptor,
      thyroid hormone receptor, estrogen receptor alpha, and glucocorticoid receptor,
      basal factors TFIIA and TBP, and transcription integrators CBP/p300 and SRC-1. In
      transient cotransfections, ASC-2, either alone or in conjunction with CBP/p300
      and SRC-1, stimulated ligand-dependent transactivation by wild type nuclear
      receptors but not mutant receptors lacking the AF2 domain. Consistent with an
      idea that ASC-2 is essential for the nuclear receptor function in vivo,
      microinjection of anti-ASC-2 antibody abrogated the ligand-dependent
      transactivation of retinoic acid receptor, and this repression was fully relieved
      by coinjection of ASC-2-expression vector. Surprisingly, ASC-2 was identical to a
      gene previously identified during a search for genes amplified and overexpressed 
      in breast and other human cancers. From these results, we concluded that ASC-2 is
      a bona fide transcription coactivator molecule of nuclear receptors, and its
      altered expression may contribute to the development of cancers.
FAU - Lee, S K
AU  - Lee SK
AD  - Center for Ligand and Transcription, Chonnam National University, Kwangju
      500-757, Korea.
FAU - Anzick, S L
AU  - Anzick SL
FAU - Choi, J E
AU  - Choi JE
FAU - Bubendorf, L
AU  - Bubendorf L
FAU - Guan, X Y
AU  - Guan XY
FAU - Jung, Y K
AU  - Jung YK
FAU - Kallioniemi, O P
AU  - Kallioniemi OP
FAU - Kononen, J
AU  - Kononen J
FAU - Trent, J M
AU  - Trent JM
FAU - Azorsa, D
AU  - Azorsa D
FAU - Jhun, B H
AU  - Jhun BH
FAU - Cheong, J H
AU  - Cheong JH
FAU - Lee, Y C
AU  - Lee YC
FAU - Meltzer, P S
AU  - Meltzer PS
FAU - Lee, J W
AU  - Lee JW
LA  - eng
SI  - GENBANK/AF177388
SI  - GENBANK/AF208227
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Ligands)
RN  - 0 (NCOA6 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nuclear Receptor Coactivators)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cloning, Molecular
MH  - Gene Amplification
MH  - Gene Expression
MH  - Gene Expression Regulation, Neoplastic
MH  - Histone Acetyltransferases
MH  - Humans
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Ligands
MH  - Molecular Sequence Data
MH  - Neoplasms/genetics
MH  - Nuclear Proteins/genetics/metabolism
MH  - Nuclear Receptor Coactivator 1
MH  - Nuclear Receptor Coactivators
MH  - Oocytes/metabolism
MH  - Protein Binding
MH  - Receptors, Cytoplasmic and Nuclear/genetics/metabolism/*physiology
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Alignment
MH  - Trans-Activators/genetics/metabolism/*physiology
MH  - Transcription Factors/genetics/metabolism/*physiology
MH  - Transcriptional Activation/*genetics
MH  - Tumor Cells, Cultured
MH  - Two-Hybrid System Techniques
MH  - Xenopus
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.48.34283 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 26;274(48):34283-93. doi: 10.1074/jbc.274.48.34283.