PMID- 10567402
OWN - NLM
STAT- MEDLINE
DCOM- 19991229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 48
DP  - 1999 Nov 26
TI  - Suppression of microphthalmia transcriptional activity by its association with
      protein kinase C-interacting protein 1 in mast cells.
PG  - 34272-6
AB  - Microphthalmia (mi) is a transcription factor that plays a major role in the
      regulation of growth and function in mast cells and melanocytes. Association of
      mi with other proteins is a critical step in the regulation of mi-mediated
      transcriptional activation. We found protein kinase C-interacting protein 1
      (PKCI) specifically associated with mi in yeast two-hybrid screening.
      Immunoprecipitation of mi from quiescent rat basophilic leukemic cells or mouse
      melanocytes resulted in the specific co-immunoprecipitation of PKCI. This
      association was significantly reduced on engagement of the surface FcepsilonRI of
      mast cells or engagement of the Kit receptor on melanocytes. Hence, cell
      activation caused disengagement of mi from PKCI. Microphthalmia was previously
      shown to activate the mouse mast cell protease 6 (mMCP-6) promoter.
      Cotransfection of mi with PKCI in NIH 3T3 fibroblasts containing an mMCP-6
      promoter-luciferase reporter demonstrated an up to 94% inhibition of mi-mediated 
      transcriptional activation. PKCI by itself, although localized in the cytosol and
      nucleus of the cells, has no known physiological function and did not demonstrate
      transcriptional activity. Its ability to suppres mi transcriptional activity in
      the transient transfected fibroblast system suggests that it can function in vivo
      as a negative regulator of mi-induced transcriptional activation.
FAU - Razin, E
AU  - Razin E
AD  - Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem 
      91120, Israel. ehudr@cc.huji.ac.il
FAU - Zhang, Z C
AU  - Zhang ZC
FAU - Nechushtan, H
AU  - Nechushtan H
FAU - Frenkel, S
AU  - Frenkel S
FAU - Lee, Y N
AU  - Lee YN
FAU - Arudchandran, R
AU  - Arudchandran R
FAU - Rivera, J
AU  - Rivera J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hint1 protein, mouse)
RN  - 0 (Microphthalmia-Associated Transcription Factor)
RN  - 0 (Mitf protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (protein kinase C-binding protein Beta15, rat)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Line
MH  - DNA-Binding Proteins/genetics/*metabolism/physiology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gene Expression Regulation
MH  - Luciferases/genetics/metabolism
MH  - Mast Cells/*metabolism
MH  - Mice
MH  - Microphthalmia-Associated Transcription Factor
MH  - Microscopy, Confocal
MH  - Nerve Tissue Proteins/genetics/*metabolism
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/analysis/genetics/metabolism
MH  - *Transcription Factors
MH  - Transcription, Genetic/*genetics
MH  - Two-Hybrid System Techniques
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.48.34272 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 26;274(48):34272-6. doi: 10.1074/jbc.274.48.34272.