PMID- 10567383
OWN - NLM
STAT- MEDLINE
DCOM- 19991229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 48
DP  - 1999 Nov 26
TI  - Molecular cloning and expression of a mouse thiamin pyrophosphokinase cDNA.
PG  - 34129-33
AB  - Thiamin pyrophosphokinase (EC 2.7.6.2) catalyzes the pyrophosphorylation of
      thiamin with adenosine 5'-triphosphate to form thiamin pyrophosphate. A mouse
      thiamin pyrophosphokinase cDNA clone (mTPK1) was isolated using a combination of 
      mouse expressed sequence tag database analysis, a two-step polymerase chain
      reaction procedure, and functional complementation screening with a Saccharomyces
      cerevisiae thiamin pyrophosphokinase-deficient mutant (thi80). The predicted
      protein contained 243 amino acid residues with a calculated molecular weight of
      27,068. When the intact mTPK1 open reading frame was expressed as a glutathione
      S-transferase fusion protein in Escherichia coli lacking thiamin
      pyrophosphokinase, marked enzyme activity was detected in the bacterial cells.
      The corresponding 2.5-kilobase pair mRNA was expressed in a tissue-dependent
      manner and was found at relatively high levels in the kidney and liver,
      indicating that the mode of expression of mTPK1 genes differs with cell type. The
      expression of mTPK1 genes in cultured mouse neuroblastoma and normal liver cells 
      was unaffected by the thiamin concentration in the medium (10 microM versus 3.0
      nM). This is the first report on identification of the primary sequence for
      mammalian thiamin pyrophosphokinase.
FAU - Nosaka, K
AU  - Nosaka K
AD  - Department of Chemistry, Kyoto Prefectural University of Medicine, Japan.
      knosaka@basic.kpu-m.ac.jp
FAU - Onozuka, M
AU  - Onozuka M
FAU - Nishino, H
AU  - Nishino H
FAU - Nishimura, H
AU  - Nishimura H
FAU - Kawasaki, Y
AU  - Kawasaki Y
FAU - Ueyama, H
AU  - Ueyama H
LA  - eng
SI  - GENBANK/AB027568
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.1.- (Thiamine Pyrophosphatase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/*genetics
MH  - Gene Expression
MH  - Genetic Complementation Test
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutation
MH  - RNA, Messenger/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Thiamine Pyrophosphatase/*genetics
MH  - Tissue Distribution
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.48.34129 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 26;274(48):34129-33. doi: 10.1074/jbc.274.48.34129.