PMID- 10567368
OWN - NLM
STAT- MEDLINE
DCOM- 19991229
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 48
DP  - 1999 Nov 26
TI  - Transcriptional regulation of the transforming growth factor-beta2 promoter by
      cAMP-responsive element-binding protein (CREB) and activating transcription
      factor-1 (ATF-1) is modulated by protein kinases and the coactivators p300 and
      CREB-binding protein.
PG  - 34020-8
AB  - Transcription of the transforming growth factor-beta2 (TGF-beta2) gene is
      dependent on a cAMP-response element/activating transcription factor (CRE/ATF)
      site that is bound by CREB and ATF-1 as well as an E-box motif that is bound by
      upstream stimulatory factors 1 and 2 (USF1 and USF2). To identify additional
      factors involved in the expression of the TGF-beta2 gene, we employed F9
      embryonal carcinoma (EC) cells, which express TGF-beta2 only after the cells
      differentiate. We show that overexpression of the transcription factors, CREB,
      ATF-1, USF1, and USF2 dramatically increases TGF-beta2 promoter activity in
      F9-differentiated cells. We further show that the coactivators p300 and CBP
      up-regulate the TGF-beta2 promoter when CREB and ATF-1 are expressed in
      conjunction with protein kinases that phosphorylate CREB on serine 133 and ATF-1 
      on serine 63. Importantly, we identify the presence of serine 133-phosphorylated 
      CREB in the nucleus of F9-differentiated cells but not in the nucleus of F9 EC
      cells. This phosphorylated form is present in whole cell extracts of both the
      parental and differentiated cells, suggesting that nuclear accumulation of serine
      133-phosphorylated CREB is regulated during differentiation of F9 EC cells and is
      likely to play an important role in the activation of the TGF-beta2 gene.
FAU - Kingsley-Kallesen, M L
AU  - Kingsley-Kallesen ML
AD  - Eppley Institute for Research in Cancer and Allied Diseases, University of
      Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA.
FAU - Kelly, D
AU  - Kelly D
FAU - Rizzino, A
AU  - Rizzino A
LA  - eng
GR  - CA 36727/CA/NCI NIH HHS/United States
GR  - CA 74771/CA/NCI NIH HHS/United States
GR  - CA 79491/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Activating Transcription Factor 1)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Upstream Stimulatory Factors)
RN  - 452VLY9402 (Serine)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Activating Transcription Factor 1
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - CREB-Binding Protein
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/metabolism
MH  - Catalytic Domain
MH  - Cell Differentiation
MH  - Cell Nucleus/metabolism
MH  - Cyclic AMP Response Element-Binding Protein/chemistry/genetics/*metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/genetics
MH  - *DNA-Binding Proteins
MH  - Enhancer Elements, Genetic
MH  - Gene Expression Regulation
MH  - Mitogen-Activated Protein Kinases/genetics/metabolism
MH  - Nuclear Proteins/genetics/physiology
MH  - Phosphorylation
MH  - *Promoter Regions, Genetic
MH  - Protein Kinases/genetics/*metabolism
MH  - Recombinant Fusion Proteins/genetics
MH  - Serine/metabolism
MH  - Trans-Activators/genetics/*physiology
MH  - Transcription Factors/genetics/*metabolism/physiology
MH  - Transcription, Genetic
MH  - Transfection
MH  - Transforming Growth Factor beta/*genetics/metabolism
MH  - Tumor Cells, Cultured/cytology/metabolism
MH  - Upstream Stimulatory Factors
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.48.34020 [doi]
AID - S0021-9258(19)53495-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 26;274(48):34020-8. doi: 10.1074/jbc.274.48.34020.