PMID- 10567359
OWN - NLM
STAT- MEDLINE
DCOM- 19991229
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 48
DP  - 1999 Nov 26
TI  - Erythropoietin induces glycosylphosphatidylinositol hydrolysis. Possible
      involvement of phospholipase c-gamma(2).
PG  - 33966-72
AB  - We showed that erythropoietin induced rapid glycosylphosphatidylinositol (GPI)
      hydrolysis and tyrosine phosphorylation of phospholipase C (PLC)-gamma(2) in
      FDC-P1 cells transfected with the wild-type erythropoietin-receptor.
      Erythropoietin-induced tyrosine phosphorylation of PLC-gamma(2) was time- and
      dose-dependent. By using FDC-P1 cells transfected with an erythropoietin receptor
      devoid of tyrosine residues, we showed that both effects required the tyrosine
      residues of intracellular domain on the erythropoietin receptor.
      Erythropoietin-activated PLC-gamma(2) hydrolyzed purified [(3)H]GPI indicating
      that GPI hydrolysis and PLC-gamma(2) activation under erythropoietin stimulation 
      were correlated. Results obtained on FDC-P1 cells transfected with erythropoietin
      receptor mutated on tyrosine residues suggest that tyrosines 343, 401, 464,
      and/or 479 are involved in erythropoietin-induced GPI hydrolysis and tyrosine
      phosphorylation of PLC-gamma(2), whereas tyrosines 429 and/or 431 seem to be
      involved in an inhibition of both effects. Thus, our results suggest that
      erythropoietin regulates GPI hydrolysis via tyrosine phosphorylation of its
      receptor and PLC-gamma(2) activation.
FAU - Boudot, C
AU  - Boudot C
AD  - Laboratoire de Biochimie, CNRS UPRES-A 6021, IFR 53 Biomolecules, UFR Sciences
      Exactes et Naturelles, BP 1039, Universite de Reims Champagne-Ardenne, F 51687
      Reims Cedex 2, France.
FAU - Petitfrere, E
AU  - Petitfrere E
FAU - Kadri, Z
AU  - Kadri Z
FAU - Chretien, S
AU  - Chretien S
FAU - Mayeux, P
AU  - Mayeux P
FAU - Haye, B
AU  - Haye B
FAU - Billat, C
AU  - Billat C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Glycosylphosphatidylinositols)
RN  - 0 (Isoenzymes)
RN  - 0 (Receptors, Erythropoietin)
RN  - 11096-26-7 (Erythropoietin)
RN  - 42HK56048U (Tyrosine)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Erythropoietin/*pharmacology
MH  - Glycosylphosphatidylinositols/*metabolism
MH  - Hydrolysis/drug effects
MH  - Isoenzymes/metabolism
MH  - Mutation
MH  - Phospholipase C gamma
MH  - Phosphorylation/drug effects
MH  - Receptors, Erythropoietin/chemistry/genetics/metabolism
MH  - Transfection
MH  - Type C Phospholipases/metabolism
MH  - Tyrosine/metabolism
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.48.33966 [doi]
AID - S0021-9258(19)53486-X [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 26;274(48):33966-72. doi: 10.1074/jbc.274.48.33966.