PMID- 10567350
OWN - NLM
STAT- MEDLINE
DCOM- 19991229
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 48
DP  - 1999 Nov 26
TI  - New mammalian selenocysteine-containing proteins identified with an algorithm
      that searches for selenocysteine insertion sequence elements.
PG  - 33888-97
AB  - Mammalian selenium-containing proteins identified thus far contain selenium in
      the form of a selenocysteine residue encoded by UGA. These proteins lack common
      amino acid sequence motifs, but 3'-untranslated regions of selenoprotein genes
      contain a common stem-loop structure, selenocysteine insertion sequence (SECIS)
      element, that is necessary for decoding UGA as selenocysteine rather than a stop 
      signal. We describe here a computer program, SECISearch, that identifies
      mammalian selenoprotein genes by recognizing SECIS elements on the basis of their
      primary and secondary structures and free energy requirements. When SECISearch
      was applied to search human dbEST, two new mammalian selenoproteins, designated
      SelT and SelR, were identified. We determined their cDNA sequences and expressed 
      them in a monkey cell line as fusion proteins with a green fluorescent protein.
      Incorporation of selenium into new proteins was confirmed by metabolic labeling
      with (75)Se, and expression of SelT was additionally documented in immunoblot
      assays. SelT and SelR did not have homology to previously characterized proteins,
      but their putative homologs were detected in various organisms. SelR homologs
      were present in every organism characterized by complete genome sequencing. The
      data suggest applicability of SECISearch for identification of new selenoprotein 
      genes in nucleotide data bases.
FAU - Kryukov, G V
AU  - Kryukov GV
AD  - Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664,
      USA.
FAU - Kryukov, V M
AU  - Kryukov VM
FAU - Gladyshev, V N
AU  - Gladyshev VN
LA  - eng
SI  - GENBANK/AF195141
SI  - GENBANK/AF195142
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (3' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (SELT protein, human)
RN  - 0 (Selenoproteins)
RN  - 0CH9049VIS (Selenocysteine)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 1.8.4.- (Methionine Sulfoxide Reductases)
SB  - IM
MH  - 3' Untranslated Regions
MH  - *Algorithms
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA, Complementary/chemistry/genetics
MH  - Expressed Sequence Tags
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Immunoblotting
MH  - Luminescent Proteins/genetics/metabolism
MH  - Mammals
MH  - Methionine Sulfoxide Reductases
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Proteins/chemistry/*genetics/metabolism
MH  - RNA, Messenger/chemistry/genetics
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Selenocysteine/*genetics
MH  - Selenoproteins
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.48.33888 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 26;274(48):33888-97. doi: 10.1074/jbc.274.48.33888.