PMID- 10567269
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 286
IP  - 5444
DP  - 1999 Nov 19
TI  - Regulation of myosin phosphatase by a specific interaction with cGMP- dependent
      protein kinase Ialpha.
PG  - 1583-7
AB  - Contraction and relaxation of smooth muscle are regulated by myosin light-chain
      kinase and myosin phosphatase through phosphorylation and dephosphorylation of
      myosin light chains. Cyclic guanosine monophosphate (cGMP)-dependent protein
      kinase Ialpha (cGKIalpha) mediates physiologic relaxation of vascular smooth
      muscle in response to nitric oxide and cGMP. It is shown here that cGKIalpha is
      targeted to the smooth muscle cell contractile apparatus by a leucine zipper
      interaction with the myosin-binding subunit (MBS) of myosin phosphatase.
      Uncoupling of the cGKIalpha-MBS interaction prevents cGMP-dependent
      dephosphorylation of myosin light chain, demonstrating that this interaction is
      essential to the regulation of vascular smooth muscle cell tone.
FAU - Surks, H K
AU  - Surks HK
AD  - Molecular Cardiology Research Institute and Cardiology Division, Department of
      Medicine, Tufts University School of Medicine and New England Medical Center,
      Boston, MA 02111, USA.
FAU - Mochizuki, N
AU  - Mochizuki N
FAU - Kasai, Y
AU  - Kasai Y
FAU - Georgescu, S P
AU  - Georgescu SP
FAU - Tang, K M
AU  - Tang KM
FAU - Ito, M
AU  - Ito M
FAU - Lincoln, T M
AU  - Lincoln TM
FAU - Mendelsohn, M E
AU  - Mendelsohn ME
LA  - eng
GR  - HL09330/HL/NHLBI NIH HHS/United States
GR  - HL55309/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Histones)
RN  - 0 (Isoenzymes)
RN  - 0 (Myosin Light Chains)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinase Type I)
RN  - EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinases)
RN  - EC 2.7.11.12 (PRKG1 protein, human)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.53 (Myosin-Light-Chain Phosphatase)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Substitution
MH  - Animals
MH  - Cells, Cultured
MH  - Cyclic GMP-Dependent Protein Kinase Type I
MH  - Cyclic GMP-Dependent Protein Kinases/chemistry/genetics/*metabolism
MH  - Histones/metabolism
MH  - Humans
MH  - Isoenzymes/chemistry/metabolism
MH  - Leucine Zippers
MH  - Muscle Contraction
MH  - Muscle Relaxation
MH  - Muscle, Smooth, Vascular/*enzymology/physiology
MH  - Mutagenesis, Site-Directed
MH  - Myosin Light Chains/*metabolism
MH  - Myosin-Light-Chain Phosphatase
MH  - Phosphoprotein Phosphatases/chemistry/*metabolism
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Rats
MH  - Recombinant Fusion Proteins/metabolism
MH  - Substrate Specificity
MH  - Transfection
MH  - Two-Hybrid System Techniques
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 8013 [pii]
AID - 10.1126/science.286.5444.1583 [doi]
PST - ppublish
SO  - Science. 1999 Nov 19;286(5444):1583-7. doi: 10.1126/science.286.5444.1583.