PMID- 10567243
OWN - NLM
STAT- MEDLINE
DCOM- 20000211
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 344 Pt 2
DP  - 1999 Dec 1
TI  - Synapsins as major neuronal Ca2+/S100A1-interacting proteins.
PG  - 577-83
AB  - The mammalian S100A1 protein can activate the invertebrate myosin-associated
      giant protein kinase twitchin in a Ca(2+)-dependent manner by more than 1000-fold
      in vitro; however, no mammalian S100-dependent protein kinases are known. In an
      attempt to identify novel mammalian Ca(2+)/S100A1-regulated protein kinases,
      brain extracts were subjected to combined Ca(2+)-dependent affinity
      chromatography with S100A1 and an ATP analogue. This resulted in the purification
      to near-homogeneity of the four major synapsin isoforms Ia, Ib, IIa and IIb. All 
      four synapsins were specifically affinity-labelled with the ATP analogue
      5'-p-fluorosulphonylbenzoyladenosine. S100A1 bound to immobilized synapsin IIa in
      BIAcore experiments in a Ca(2+)-dependent and Zn(2+)-enhanced manner with
      submicromolar affinity; this interaction could be competed for with synthetic
      peptides of the proposed S100A1-binding sites of synapsin. Double-labelling
      confocal immunofluorescence microscopy demonstrated that synapsins and S100A1 are
      both present in the soma and neurites of PC12 cells, indicating their potential
      to interact in neurons in vivo.
FAU - Heierhorst, J
AU  - Heierhorst J
AD  - St. Vincent's Institute of Medical Research, 41 Victoria Parade, Fitzroy, VIC
      3065, Australia. heier@ariel.its.unimelb.edu.au
FAU - Mitchelhill, K I
AU  - Mitchelhill KI
FAU - Mann, R J
AU  - Mann RJ
FAU - Tiganis, T
AU  - Tiganis T
FAU - Czernik, A J
AU  - Czernik AJ
FAU - Greengard, P
AU  - Greengard P
FAU - Kemp, B E
AU  - Kemp BE
LA  - eng
GR  - MH39327/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (S100 Proteins)
RN  - 0 (S100A1 protein)
RN  - 0 (Synapsins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/analogs & derivatives/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain Chemistry
MH  - Calcium/*metabolism
MH  - Calcium-Binding Proteins/*metabolism
MH  - Cell Compartmentation
MH  - Chromatography, Affinity
MH  - Molecular Sequence Data
MH  - Neurites/chemistry
MH  - Neurons/*chemistry/metabolism
MH  - PC12 Cells
MH  - Protein Binding
MH  - Protein Isoforms/metabolism
MH  - Rats
MH  - S100 Proteins
MH  - Sequence Analysis, Protein
MH  - Synapsins/isolation & purification/*metabolism
PMC - PMC1220678
EDAT- 1999/11/24 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/11/24 09:00
PHST- 1999/11/24 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/11/24 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 1999 Dec 1;344 Pt 2:577-83.