PMID- 10564823
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 240
IP  - 1
DP  - 1999 Nov 15
TI  - Expression of rat BTG(3) gene, Rbtg3, is regulated by redox changes.
PG  - 165-73
AB  - The Rbtg3 gene was isolated by PCR (polymerase chain reaction) cloning from the
      cDNA library of Rat1 fibroblasts that were stimulated with TPA
      (12-O-tetradecanoylphorbol-13-acetate) or various growth factors for 3h and was
      found to be a rat homologue of mouse BTG3 and human ANA genes. The Rbtg3 gene had
      unique DNA sequences in the 5'-UTR and 3'-UTR that contained four ATTTA and one
      TTATTTA(T/A)(T/A) nonamer motif, and also a polyA addition site. Nucleotide
      homology of Rbtg3 with BTG3 and ANA was 88.5 and 76.6%, respectively. Expression 
      of Rbtg3 was investigated in SD rats as well as cell lines derived from
      mouse--SW3T3, NIH3T3 fibroblasts--and rat--Rat1, 3Y1 fibroblasts and PC12--cells.
      Rbtg3 was highly expressed in brain but barely in lung, kidney, thymus and
      spleen. The constitutive expression level was high in SW3T3, Rat1 and 3Y1
      fibroblasts, but very low in NIH3T3 fibroblast and PC12 cells. However, in all
      cells tested, Rbtg3 was proved to be one of the primary response genes
      superinduced by TPA (50ng/ml)+cycloheximide (CHX, 10 microgram/ml). Expression of
      Rbtg3 was induced by H(2)O(2) (500mM) up to fourfold in PC12 cells and was
      blocked by pretreatment of NAC (N-acetyl-L-cysteine, 10mM). The induction was
      ninefold in 3Y1 fibroblasts by menadione (25mM) treatment for 1h, whereas it was 
      reduced to a third of the control level in SW3T3 fibroblast by the same
      treatment. Rbtg3 was not expressed in NIH3T3 cells but minimally regulated by
      redox changes as compared with rapid and strong induction of TIS21/BTG2 mRNAs
      after TPA or H(2)O(2) stimulation. The above results indicate that Rbtg3 is one
      of many redox-regulated genes as well as a primary response gene.
FAU - Seo, M S
AU  - Seo MS
AD  - Department of Biochemistry, Ajou University School of Medicine, Suwon, South
      Korea.
FAU - Lee, M S
AU  - Lee MS
FAU - Lim, I K
AU  - Lim IK
LA  - eng
SI  - GENBANK/AF087037
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Btg2 protein, mouse)
RN  - 0 (Btg3 protein, rat)
RN  - 0 (DNA, Complementary)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 63231-63-0 (RNA)
RN  - 98600C0908 (Cycloheximide)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - 3' Untranslated Regions
MH  - 3T3 Cells
MH  - 5' Untranslated Regions
MH  - Acetylcysteine/pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Brain/metabolism
MH  - Cell Line
MH  - Cycloheximide/pharmacology
MH  - DNA, Complementary/chemistry/genetics
MH  - Free Radical Scavengers/pharmacology
MH  - Gene Expression Regulation/drug effects/*genetics
MH  - *Genes, Tumor Suppressor
MH  - Hydrogen Peroxide/pharmacology
MH  - Immediate-Early Proteins/drug effects/genetics/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Oxidation-Reduction
MH  - PC12 Cells
MH  - Proteins/*genetics
MH  - RNA/genetics/metabolism
MH  - RNA, Messenger/drug effects/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Suppressor Proteins
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0378-1119(99)00415-1 [pii]
AID - 10.1016/s0378-1119(99)00415-1 [doi]
PST - ppublish
SO  - Gene. 1999 Nov 15;240(1):165-73. doi: 10.1016/s0378-1119(99)00415-1.