PMID- 10564755
OWN - NLM
STAT- MEDLINE
DCOM- 20000120
LR  - 20190910
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 65
IP  - 1
DP  - 1999 Dec 1
TI  - The complete nucleotide sequence of RNA2 of blackcurrant reversion nepovirus.
PG  - 87-92
AB  - The complete nucleotide sequence of blackcurrant reversion nepovirus (BRV) RNA2
      was determined from cDNA clones. RNA2 was 6400 nucleotides (nt) in length
      excluding the 3' poly(A)-tail. It contained a single open reading frame of 4878
      nts encoding a polypeptide of 1626 amino acids with a calculated M(r) of 178
      inverted question mark omitted inverted question mark860. The genome organization
      of BRV RNA2 was similar to that of other nepoviruses, especially those with a
      large RNA2. The coat protein (CP) was located in the C-terminal region of the
      large polyprotein and contained amino acid motifs conserved among nepovirus CPs. 
      Sequence comparisons revealed a proline (P) residue surrounded by hydrophobic
      amino acid residues located upstream of the CP. This P motif is conserved among
      the putative movement proteins of nepo-, como-, caulimo- and capilloviruses. An
      N-terminal domain of 350 amino acids of RNA2-encoded polyprotein shared 34 and
      35% sequence identity with the N-terminal domains of tomato ringspot nepovirus
      RNA1- and RNA2-encoded polyproteins, respectively. Sequence identities between
      the N-terminal domains of BRV RNA2 and other nepoviral RNA2s were less than 20%; 
      no common N-terminal motif was found.
FAU - Latvala-Kilby, S
AU  - Latvala-Kilby S
AD  - Laboratory of Plant Physiology and Molecular Biology, University of Turku, Turku,
      FIN-20014, Finland. satu.latvala @utu.fi
FAU - Lehto, K
AU  - Lehto K
LA  - eng
SI  - GENBANK/AF020051
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - *Genome, Viral
MH  - Magnoliopsida/*virology
MH  - Molecular Sequence Data
MH  - Nepovirus/*genetics
MH  - RNA, Viral/*analysis
MH  - Sequence Homology, Amino Acid
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0168170299001057 [pii]
AID - 10.1016/s0168-1702(99)00105-7 [doi]
PST - ppublish
SO  - Virus Res. 1999 Dec 1;65(1):87-92. doi: 10.1016/s0168-1702(99)00105-7.