PMID- 10564580
OWN - NLM
STAT- MEDLINE
DCOM- 20000207
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 27
IP  - 1
DP  - 2000 Jan
TI  - Loss of expression of the DRR 1 gene at chromosomal segment 3p21.1 in renal cell 
      carcinoma.
PG  - 1-10
AB  - Consistent deletion of DNA sequences in chromosomal band 3p21 observed in a
      variety of human tumors suggests the presence of one or more tumor suppressor
      genes within this region. Previously, we reported on the construction of two
      distinct cosmid contigs and our identification of several new genes within
      3p21.1. In our search for tumor suppressor genes from this region, we have cloned
      a gene that we have called DRR 1 (downregulated in renal cell carcinoma). The
      gene was first mapped to 3p21.1 by fluorescence in situ hybridization analysis.
      Further analysis of yeast artificial chromosome clones in 3p14.2-p21.1 refined
      its localization. DRR 1 spans about 10 Kb of genomic DNA with a 3.5-Kb mature
      transcript. The putative protein encoded by this gene is 144 amino acids and
      includes a nuclear localization signal and a coiled domain. The gene showed loss 
      of expression in eight of eight renal cell carcinoma cell lines, one of seven
      ovarian cancer cell lines, one of one cervical cancer cell line, one of one
      gastric cancer cell line, and one of one non-small-cell lung cancer cell line.
      Southern blot analysis did not show any altered bands, indicating that gross
      structural changes or deletions did not cause the loss of expression. This gene
      was also found to have reduced expression in 23 of 34 paired primary renal cell
      carcinomas. Mutational analysis detected three polymorphic sites within the gene,
      but no point mutations were identified in the 34 primary tumors. However, we did 
      detect base substitutions in 4 of 12 cell lines that had undetectable expression 
      of the gene. We also transfected the gene into DRR 1-negative cell lines and
      observed clear growth retardation. Our results suggest that loss of expression of
      the DRR 1 gene may play an important role in the development of renal cell
      carcinoma and possibly other tumors. Genes Chromosomes Cancer 27:1-10, 2000.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Wang, L
AU  - Wang L
AD  - Division of Experimental Pathology, Department of Laboratory Medicine and
      Pathology, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Darling, J
AU  - Darling J
FAU - Zhang, J S
AU  - Zhang JS
FAU - Liu, W
AU  - Liu W
FAU - Qian, J
AU  - Qian J
FAU - Bostwick, D
AU  - Bostwick D
FAU - Hartmann, L
AU  - Hartmann L
FAU - Jenkins, R
AU  - Jenkins R
FAU - Bardenhauer, W
AU  - Bardenhauer W
FAU - Schutte, J
AU  - Schutte J
FAU - Opalka, B
AU  - Opalka B
FAU - Smith, D I
AU  - Smith DI
LA  - eng
SI  - GENBANK/AF089853
GR  - CA48031/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA, Complementary)
RN  - 0 (FAM107A protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Carcinoma, Renal Cell/*genetics/metabolism
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Cloning, Molecular
MH  - DNA Mutational Analysis
MH  - DNA, Complementary
MH  - Down-Regulation/genetics
MH  - Female
MH  - Gene Expression
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - *Nuclear Proteins
MH  - Ovarian Neoplasms/genetics/metabolism
MH  - Protein Biosynthesis
MH  - Proteins/chemistry/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1002/(SICI)1098-2264(200001)27:1<1::AID-GCC1>3.0.CO;2-6 [pii]
AID - 10.1002/(sici)1098-2264(200001)27:1<1::aid-gcc1>3.0.co;2-6 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Jan;27(1):1-10. doi:
      10.1002/(sici)1098-2264(200001)27:1<1::aid-gcc1>3.0.co;2-6.