PMID- 10564375
OWN - NLM
STAT- MEDLINE
DCOM- 19991208
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 11
IP  - 10
DP  - 1999 Oct
TI  - Differential expression of the presynaptic cytomatrix protein bassoon among
      ribbon synapses in the mammalian retina.
PG  - 3683-93
AB  - Bassoon is a 420-kDa presynaptic protein which is highly concentrated at the
      active zones of nerve terminals of conventional synapses, both excitatory
      glutamatergic and inhibitory GABAergic, in rat brain. It is thought to be
      involved in the organization of the cytomatrix at the site of neurotransmitter
      release. In the retina, there are two structurally and functionally distinct
      types of synapses: ribbon and conventional synapses. Antibodies against bassoon
      were applied to sections of rat and rabbit retina. Strong punctate
      immunofluorescence was found in the outer and inner plexiform layers. Using pre- 
      and post-embedding immunostaining and electron microscopy, bassoon was localized 
      in the outer plexiform layer at ribbon synapses formed by rods and cones but was 
      absent from basal synaptic contacts formed by cones. In the inner plexiform layer
      a different picture emerged. As in the brain, bassoon was found at conventional
      inhibitory GABAergic synapses, made by amacrine cells, but it was absent from the
      bipolar cell ribbon synapses. These data demonstrate differences in the molecular
      composition of the presynaptic apparatuses of outer and inner plexiform layer
      ribbon synapses. Thus, differential equipment with cytomatrix proteins may
      account for the functional differences observed between the two types of ribbon
      synapses in the retina.
FAU - Brandstatter, J H
AU  - Brandstatter JH
AD  - Max-Planck-Institut fur Hirnforschung, Abteilung fur Neuroanatomie, Frankfurt am 
      Main, Germany. brandstaett@mpih-frankfurt.mpg.de
FAU - Fletcher, E L
AU  - Fletcher EL
FAU - Garner, C C
AU  - Garner CC
FAU - Gundelfinger, E D
AU  - Gundelfinger ED
FAU - Wassle, H
AU  - Wassle H
LA  - eng
GR  - P50 HD32901/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Biomarkers)
RN  - 0 (Bsn protein, rat)
RN  - 0 (GABA-C receptor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, GABA)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Exocytosis/physiology
MH  - Fluorescent Antibody Technique
MH  - Mammals
MH  - Microscopy, Immunoelectron
MH  - Nerve Tissue Proteins/*analysis/*biosynthesis
MH  - Photoreceptor Cells, Vertebrate/*chemistry/metabolism/ultrastructure
MH  - Presynaptic Terminals/*chemistry/*metabolism/ultrastructure
MH  - Rabbits
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, GABA/analysis
MH  - Zinc Fingers/physiology
EDAT- 1999/11/17 00:00
MHDA- 1999/11/17 00:01
CRDT- 1999/11/17 00:00
PHST- 1999/11/17 00:00 [pubmed]
PHST- 1999/11/17 00:01 [medline]
PHST- 1999/11/17 00:00 [entrez]
AID - ejn793 [pii]
AID - 10.1046/j.1460-9568.1999.00793.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 1999 Oct;11(10):3683-93. doi: 10.1046/j.1460-9568.1999.00793.x.