PMID- 10564259
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 11
DP  - 1999 Nov
TI  - Mechanisms of G2 arrest in response to overexpression of p53.
PG  - 3607-22
AB  - Overexpression of p53 causes G2 arrest, attributable in part to the loss of CDC2 
      activity. Transcription of cdc2 and cyclin B1, determined using reporter
      constructs driven by the two promoters, was suppressed in response to the
      induction of p53. Suppression requires the regions -287 to -123 of the cyclin B1 
      promoter and -104 to -74 of the cdc2 promoter. p53 did not affect the inhibitory 
      phosphorylations of CDC2 at threonine 14 or tyrosine 15 or the activity of the
      cyclin-dependent kinase that activates CDC2 by phosphorylating it at threonine
      161. Overexpression of p53 may also interfere with the accumulation of
      CDC2/cyclin B1 in the nucleus, required for cells to enter mitosis. Constitutive 
      expression of cyclin B1, alone or in combination with the constitutively active
      CDC2 protein T14A Y15F, did not reverse p53-dependent G2 arrest. However,
      targeting cyclin B1 to the nucleus in cells also expressing CDC2 T14A Y15F did
      overcome this arrest. It is likely that several distinct pathways contribute to
      p53-dependent G2 arrest.
FAU - Taylor, W R
AU  - Taylor WR
AD  - Department of Molecular Biology, Lerner Research Institute, The Cleveland Clinic 
      Foundation, Cleveland, Ohio 44195, USA.
FAU - DePrimo, S E
AU  - DePrimo SE
FAU - Agarwal, A
AU  - Agarwal A
FAU - Agarwal, M L
AU  - Agarwal ML
FAU - Schonthal, A H
AU  - Schonthal AH
FAU - Katula, K S
AU  - Katula KS
FAU - Stark, G R
AU  - Stark GR
LA  - eng
GR  - R01 GM049345/GM/NIGMS NIH HHS/United States
GR  - GM-49345/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (CCNB1 protein, human)
RN  - 0 (Cyclin B)
RN  - 0 (Cyclin B1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 500-44-7 (Mimosine)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
RN  - EC 2.7.11.22 (cyclin-dependent kinase-activating kinase)
SB  - IM
MH  - Apoptosis/*genetics
MH  - CDC2 Protein Kinase/genetics
MH  - Cell Cycle/drug effects
MH  - Cell Line
MH  - Cell Nucleus/genetics
MH  - Cyclin B/genetics
MH  - Cyclin B1
MH  - *Cyclin-Dependent Kinases
MH  - DNA/biosynthesis
MH  - Flow Cytometry
MH  - G2 Phase/*genetics
MH  - Gene Expression Regulation
MH  - Humans
MH  - Microscopy, Video
MH  - Mimosine/pharmacology
MH  - Mitosis
MH  - Phosphorylation
MH  - Promoter Regions, Genetic
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - RNA, Messenger/metabolism
MH  - Tumor Suppressor Protein p53/*metabolism
PMC - PMC25646
EDAT- 1999/11/17 00:00
MHDA- 1999/11/17 00:01
CRDT- 1999/11/17 00:00
PHST- 1999/11/17 00:00 [pubmed]
PHST- 1999/11/17 00:01 [medline]
PHST- 1999/11/17 00:00 [entrez]
AID - 10.1091/mbc.10.11.3607 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Nov;10(11):3607-22. doi: 10.1091/mbc.10.11.3607.