PMID- 10564087
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20180925
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 5
DP  - 1999 Nov
TI  - Identification of an acid-activated Cl(-) channel from human skeletal muscles.
PG  - C948-54
LID - 10.1152/ajpcell.1999.277.5.C948 [doi]
AB  - ClC-4 gene was isolated as a putative Cl(-) channel. Due to a lack of functional 
      expression of ClC-4, its physiological role remains unknown. We isolated a human 
      ClC-4 clone (hClC-4sk) from human skeletal muscles and stably transfected it to
      Chinese hamster ovary cells. Whole cell patch-clamp studies showed that the
      hClC-4sk channel was activated by external acidic pH and inhibited by DIDS. It
      passed a strong outward Cl(-) current with a permeability sequence of I(-) >
      Cl(-) > F(-). The hClC-4sk has consensus sites for phosphorylation by protein
      kinase A (PKA); however, stimulation of PKA had no effect on the currents.
      hClC-4sk mRNA was expressed in excitable tissues, such as heart, brain, and
      skeletal muscle. These functional characteristics of hClC-4sk provide a clue to
      its physiological role in excitable cells.
FAU - Kawasaki, M
AU  - Kawasaki M
AD  - Second Department of Internal Medicine, Tokyo Medical and Dental University,
      Tokyo 113-8519, Japan.
FAU - Fukuma, T
AU  - Fukuma T
FAU - Yamauchi, K
AU  - Yamauchi K
FAU - Sakamoto, H
AU  - Sakamoto H
FAU - Marumo, F
AU  - Marumo F
FAU - Sasaki, S
AU  - Sasaki S
LA  - eng
SI  - GENBANK/AB019432
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Acids)
RN  - 0 (CLCN4 protein, human)
RN  - 0 (Chloride Channels)
RN  - 0 (Chlorides)
RN  - 0 (DNA, Complementary)
RN  - 1F7A44V6OU (Colforsin)
RN  - Q1O6DSW23R (4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid)
SB  - IM
MH  - 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid/pharmacology
MH  - Acids/*pharmacology
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - Chloride Channels/*genetics/metabolism
MH  - Chlorides/metabolism
MH  - Cloning, Molecular
MH  - Colforsin/pharmacology
MH  - Cricetinae
MH  - DNA, Complementary/isolation & purification
MH  - Extracellular Space/metabolism
MH  - Gene Expression/physiology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Ion Channel Gating/*drug effects
MH  - Mesocricetus
MH  - Molecular Sequence Data
MH  - Muscle, Skeletal/*chemistry/physiology
MH  - Patch-Clamp Techniques
MH  - Transfection
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1152/ajpcell.1999.277.5.C948 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Nov;277(5):C948-54. doi: 10.1152/ajpcell.1999.277.5.C948.