PMID- 10564083
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20180925
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 5
DP  - 1999 Nov
TI  - Mouse K-Cl cotransporter KCC1: cloning, mapping, pathological expression, and
      functional regulation.
PG  - C899-912
LID - 10.1152/ajpcell.1999.277.5.C899 [doi]
AB  - Although K-Cl cotransporter (KCC1) mRNA is expressed in many tissues, K-Cl
      cotransport activity has been measured in few cell types, and detection of
      endogenous KCC1 polypeptide has not yet been reported. We have cloned the mouse
      erythroid KCC1 (mKCC1) cDNA and its flanking genomic regions and mapped the mKCC1
      gene to chromosome 8. Three anti-peptide antibodies raised against recombinant
      mKCC1 function as immunoblot and immunoprecipitation reagents. The tissue
      distributions of mKCC1 mRNA and protein are widespread, and mKCC1 RNA is
      constitutively expressed during erythroid differentiation of ES cells. KCC1
      polypeptide or related antigen is present in erythrocytes of multiple species in 
      which K-Cl cotransport activity has been documented. Erythroid KCC1 polypeptide
      abundance is elevated in proportion to reticulocyte counts in
      density-fractionated cells, in bleeding-induced reticulocytosis, in mouse models 
      of sickle cell disease and thalassemia, and in the corresponding human disorders.
      mKCC1-mediated uptake of (86)Rb into Xenopus oocytes requires extracellular
      Cl(-), is blocked by the diuretic R(+)-[2-n-butyl-6,7-dichloro-2-cyclopentyl-2,
      3-dihydro-1-oxo-1H-indenyl-5-yl-)oxy]acetic acid, and exhibits an erythroid
      pattern of acute regulation, with activation by hypotonic swelling,
      N-ethylmaleimide, and staurosporine and inhibition by calyculin and okadaic acid.
      These reagents and findings will expedite studies of KCC1 structure-function
      relationships and of the pathobiology of KCC1-mediated K-Cl cotransport.
FAU - Su, W
AU  - Su W
AD  - Molecular Medicine and Renal Units, Beth Israel Deaconess Medical Center, Boston 
      02215, USA.
FAU - Shmukler, B E
AU  - Shmukler BE
FAU - Chernova, M N
AU  - Chernova MN
FAU - Stuart-Tilley, A K
AU  - Stuart-Tilley AK
FAU - de Franceschi, L
AU  - de Franceschi L
FAU - Brugnara, C
AU  - Brugnara C
FAU - Alper, S L
AU  - Alper SL
LA  - eng
SI  - GENBANK/AF116526
SI  - GENBANK/AF121118
SI  - GENBANK/AF121128
GR  - DK-34854/DK/NIDDK NIH HHS/United States
GR  - DK-51059/DK/NIDDK NIH HHS/United States
GR  - HL-15157/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (3' Untranslated Regions)
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Carrier Proteins)
RN  - 0 (Chlorides)
RN  - 0 (DNA, Complementary)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Symporters)
RN  - 0 (potassium-chloride symporters)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - 3' Untranslated Regions/genetics
MH  - 5' Untranslated Regions/genetics
MH  - Amino Acid Sequence
MH  - Anemia, Sickle Cell/*metabolism/pathology
MH  - Animals
MH  - Antibody Specificity
MH  - Base Sequence
MH  - Biological Transport/genetics
MH  - Carrier Proteins/*genetics/immunology/*metabolism
MH  - Chlorides/pharmacokinetics
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Cross Reactions
MH  - DNA, Complementary
MH  - Erythrocytes/chemistry/cytology/metabolism
MH  - Gene Expression Regulation/physiology
MH  - Glycosylation
MH  - Humans
MH  - Kidney/cytology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Oocytes/physiology
MH  - Potassium/pharmacokinetics
MH  - Precipitin Tests
MH  - Protein Biosynthesis/physiology
MH  - RNA, Messenger/analysis
MH  - Rabbits
MH  - Rats
MH  - *Symporters
MH  - Thalassemia/*metabolism/pathology
MH  - Transfection
MH  - Xenopus
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1152/ajpcell.1999.277.5.C899 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Nov;277(5):C899-912. doi: 10.1152/ajpcell.1999.277.5.C899.