PMID- 10562553
OWN - NLM
STAT- MEDLINE
DCOM- 20000120
LR  - 20081121
IS  - 0261-4189 (Print)
IS  - 0261-4189 (Linking)
VI  - 18
IP  - 22
DP  - 1999 Nov 15
TI  - Constitutive silencing of IFN-beta promoter is mediated by NRF
      (NF-kappaB-repressing factor), a nuclear inhibitor of NF-kappaB.
PG  - 6415-25
AB  - Transcriptional regulation of the interferon-beta (IFN-beta) gene is
      characterized by strict constitutive repression and virus-specific activation.
      Previous studies have shown that the IFN-beta promoter is constitutively
      repressed by a negative regulatory element (NRE). Isolated NRE acts as a
      constitutive and position-independent silencer on the NF-kappaB-binding sites.
      Here, we describe the identification and functional characterization of the
      NRE-binding protein, called NRF (NF-kappaB-repressing factor), which abolishes
      the transcriptional activity of the bordering NF-kappaB- binding sites by a
      distance-independent mechanism. Deletion studies show that a minimal repression
      domain of NRF is sufficient to exert its inhibitory effect. In vitro, NF-kappaB
      proteins bind to purified NRF by a direct protein-protein interaction. We
      demonstrate that NRF is a ubiquitous and constitutive nuclear protein. In
      fibroblasts, the expression of the NRF antisense RNA releases the endogenous
      IFN-beta gene transcription. Our data strongly suggest that the NRF-mediated
      inhibition of NF-kappaB is a critical component of the IFN-beta gene silencing
      prior to viral infection.
FAU - Nourbakhsh, M
AU  - Nourbakhsh M
AD  - Department of Gene Regulation and Differentiation, GBF-National Research
      Institute for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany.
FAU - Hauser, H
AU  - Hauser H
LA  - eng
SI  - GENBANK/AJ011812
PT  - Journal Article
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NKRF protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cell Line
MH  - Cell Nucleus/*metabolism
MH  - Cloning, Molecular
MH  - DNA-Binding Proteins/metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - HeLa Cells
MH  - Humans
MH  - Interferon-beta/biosynthesis/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Organ Specificity
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Recombinant Proteins/biosynthesis
MH  - Repressor Proteins/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Transfection
PMC - PMC1171704
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1093/emboj/18.22.6415 [doi]
PST - ppublish
SO  - EMBO J. 1999 Nov 15;18(22):6415-25. doi: 10.1093/emboj/18.22.6415.