PMID- 10562431
OWN - NLM
STAT- MEDLINE
DCOM- 19991220
LR  - 20141120
IS  - 0003-9861 (Print)
IS  - 0003-9861 (Linking)
VI  - 372
IP  - 1
DP  - 1999 Dec 1
TI  - Association of stomatin (band 7.2b) with Glut1 glucose transporter.
PG  - 173-8
AB  - Employing a monoclonal antibody directed against the C-terminal peptide of
      glucose transporter molecule 1 (Glut1), we identified a approximately 30-kDa
      polypeptide which coimmunoprecipitated with Glut1 from sample of human red blood 
      cells (RBC) membranes. The approximately 30-kDa polypeptide reacted with an
      antibody directed against stomatin, an integral plasma membrane protein which is 
      also present at a high abundance in the human RBC plasma membrane. Likewise,
      employing anti-stomatin antibody, we found that Glut1 coimmunoprecipitated with
      stomatin from samples of RBC membranes. However, neither band 3, which is the
      most abundant integral membrane protein in the RBC, nor actin
      coimmunoprecipitated with Glut1, indicating a specific interaction between Glut1 
      and stomatin. Similar to the results obtained in the RBC, Glut1 and stomatin
      immunoprecipitated with each other in lysates of Clone 9 cells, a rat liver cell 
      line in which Glut1 is expressed at approximately 1/200 the level present in RBC.
      Employing conditions that resulted in immunoprecipitation of approximately 10% of
      Glut1 in RBC membranes led to a approximately 3% coimmunoprecipitation of
      stomatin. A mixed population of Clone 9 cells stably transfected with a plasmid
      overexpressing the mouse stomatin exhibited 30 +/- 3% reduction in the basal rate
      of glucose transport compared to control cells or cells stably transfected with
      the empty vector. The above results suggest that stomatin is closely associated
      with Glut1 in the plasma membrane and that overexpression of stomatin results in 
      a depression in the basal rate of glucose transport.
CI  - Copyright 1999 Academic Press.
FAU - Zhang, J Z
AU  - Zhang JZ
AD  - Departments of Medicine and of Physiology and Biophysics, Case Western Reserve
      University, Cleveland, Ohio, 44106-4951, USA.
FAU - Hayashi, H
AU  - Hayashi H
FAU - Ebina, Y
AU  - Ebina Y
FAU - Prohaska, R
AU  - Prohaska R
FAU - Ismail-Beigi, F
AU  - Ismail-Beigi F
LA  - eng
GR  - DK45945/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Blood Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Epb7.2 protein, mouse)
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Membrane Proteins)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (SLC2A1 protein, human)
RN  - 0 (STOM protein, human)
RN  - 0 (Slc2a1 protein, mouse)
RN  - 0 (Slc2a1 protein, rat)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Base Sequence
MH  - Biological Transport, Active
MH  - Blood Proteins/chemistry/isolation & purification/*metabolism
MH  - Cell Line
MH  - DNA Primers/genetics
MH  - Erythrocyte Membrane/chemistry
MH  - Glucose/metabolism
MH  - Glucose Transporter Type 1
MH  - Humans
MH  - In Vitro Techniques
MH  - *Membrane Proteins
MH  - Mice
MH  - Monosaccharide Transport Proteins/chemistry/isolation & purification/*metabolism
MH  - Precipitin Tests
MH  - Rats
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1006/abbi.1999.1489 [doi]
AID - S0003-9861(99)91489-3 [pii]
PST - ppublish
SO  - Arch Biochem Biophys. 1999 Dec 1;372(1):173-8. doi: 10.1006/abbi.1999.1489.