PMID- 10562324
OWN - NLM
STAT- MEDLINE
DCOM- 19991209
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 190
IP  - 10
DP  - 1999 Nov 15
TI  - Identification and molecular characterization of NKp30, a novel triggering
      receptor involved in natural cytotoxicity mediated by human natural killer cells.
PG  - 1505-16
AB  - Two major receptors involved in human natural cytotoxicity, NKp46 and NKp44, have
      recently been identified. However, experimental evidence suggested the existence 
      of additional such receptor(s). In this study, by the generation of monoclonal
      antibodies (mAbs), we identified NKp30, a novel 30-kD triggering receptor
      selectively expressed by all resting and activated human natural killer (NK)
      cells. Although mAb-mediated cross-linking of NKp30 induces strong NK cell
      activation, mAb-mediated masking inhibits the NK cytotoxicity against normal or
      tumor target cells. NKp30 cooperates with NKp46 and/or NKp44 in the induction of 
      NK-mediated cytotoxicity against the majority of target cells, whereas it
      represents the major triggering receptor in the killing of certain tumors. This
      novel receptor is associated with CD3zeta chains that become tyrosine
      phosphorylated upon sodium pervanadate treatment of NK cells. Molecular cloning
      of NKp30 cDNA revealed a member of the immunoglobulin superfamily, characterized 
      by a single V-type domain and a charged residue in the transmembrane portion.
      Moreover, we show that NKp30 is encoded by the previously identified 1C7 gene,
      for which the function and the cellular distribution of the putative product were
      not identified in previous studies.
FAU - Pende, D
AU  - Pende D
AD  - Istituto Nazionale per la Ricerca sul Cancro, 16132 Genova, Italy.
FAU - Parolini, S
AU  - Parolini S
FAU - Pessino, A
AU  - Pessino A
FAU - Sivori, S
AU  - Sivori S
FAU - Augugliaro, R
AU  - Augugliaro R
FAU - Morelli, L
AU  - Morelli L
FAU - Marcenaro, E
AU  - Marcenaro E
FAU - Accame, L
AU  - Accame L
FAU - Malaspina, A
AU  - Malaspina A
FAU - Biassoni, R
AU  - Biassoni R
FAU - Bottino, C
AU  - Bottino C
FAU - Moretta, L
AU  - Moretta L
FAU - Moretta, A
AU  - Moretta A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (NCR1 protein, human)
RN  - 0 (NCR2 protein, human)
RN  - 0 (Natural Cytotoxicity Triggering Receptor 1)
RN  - 0 (Natural Cytotoxicity Triggering Receptor 2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - COS Cells
MH  - Cloning, Molecular
MH  - *Cytotoxicity, Immunologic
MH  - Humans
MH  - Killer Cells, Natural/*immunology
MH  - Natural Cytotoxicity Triggering Receptor 1
MH  - Natural Cytotoxicity Triggering Receptor 2
MH  - RNA, Messenger/analysis
MH  - Receptors, Immunologic/*analysis/genetics/physiology
MH  - Tumor Cells, Cultured
PMC - PMC2195691
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1084/jem.190.10.1505 [doi]
PST - ppublish
SO  - J Exp Med. 1999 Nov 15;190(10):1505-16. doi: 10.1084/jem.190.10.1505.