PMID- 10562278
OWN - NLM
STAT- MEDLINE
DCOM- 19991217
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 147
IP  - 4
DP  - 1999 Nov 15
TI  - Rab6 coordinates a novel Golgi to ER retrograde transport pathway in live cells.
PG  - 743-60
AB  - We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated
      GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6
      defined highly dynamic transport carriers (TCs) translocating from the Golgi to
      the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde cargo, the 
      wild-type Shiga toxin B-fragment (STB), during STB transport from the Golgi to
      the endoplasmic reticulum (ER). FP-Rab6 TCs associated intimately with the ER,
      and STB entered the ER via specialized peripheral regions that accumulated
      FP-Rab6. Microinjection of antibodies that block coatomer protein I (COPI)
      function inhibited trafficking of a KDEL-receptor FP-fusion, but not FP-Rab6.
      Additionally, markers of COPI-dependent recycling were excluded from FP-Rab6/STB 
      TCs. Overexpression of Rab6:GDP (T27N mutant) using T7 vaccinia inhibited
      toxicity of Shiga holotoxin, but did not alter STB transport to the Golgi or
      Golgi morphology. Taken together, our results indicate Rab6 regulates a novel
      Golgi to ER transport pathway.
FAU - White, J
AU  - White J
AD  - Light Microscopy Group, European Molecular Biology Laboratory, Meyerhofstrasse 1,
      D-69117 Heidelberg, Germany. jwhite@embl-heidelberg.de
FAU - Johannes, L
AU  - Johannes L
FAU - Mallard, F
AU  - Mallard F
FAU - Girod, A
AU  - Girod A
FAU - Grill, S
AU  - Grill S
FAU - Reinsch, S
AU  - Reinsch S
FAU - Keller, P
AU  - Keller P
FAU - Tzschaschel, B
AU  - Tzschaschel B
FAU - Echard, A
AU  - Echard A
FAU - Goud, B
AU  - Goud B
FAU - Stelzer, E H
AU  - Stelzer EH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Bacterial Toxins)
RN  - 0 (KDEL receptor)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Rab6 protein)
RN  - 0 (Receptors, Peptide)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Shiga Toxins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
EIN - J Cell Biol 2000 Jan 10;148(1):followi
MH  - Animals
MH  - Bacterial Toxins/toxicity
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Endoplasmic Reticulum/drug effects/*metabolism/ultrastructure
MH  - Golgi Apparatus/drug effects/*metabolism/ultrastructure
MH  - Green Fluorescent Proteins
MH  - HeLa Cells
MH  - Humans
MH  - Luminescent Proteins/genetics/metabolism
MH  - Microscopy, Confocal
MH  - Microscopy, Immunoelectron
MH  - Receptors, Peptide/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Shiga Toxins
MH  - Transfection
MH  - rab GTP-Binding Proteins/genetics/*metabolism
PMC - PMC2156170
EDAT- 1999/11/24 09:00
MHDA- 2000/03/25 09:00
CRDT- 1999/11/24 09:00
PHST- 1999/11/24 09:00 [pubmed]
PHST- 2000/03/25 09:00 [medline]
PHST- 1999/11/24 09:00 [entrez]
AID - 10.1083/jcb.147.4.743 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Nov 15;147(4):743-60. doi: 10.1083/jcb.147.4.743.