PMID- 10561590
OWN - NLM
STAT- MEDLINE
DCOM- 20021203
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 266
IP  - 2
DP  - 1999 Dec
TI  - Identification of a CK2 phosphorylation site in mdm2.
PG  - 493-501
AB  - Mdm2 is a cellular oncoprotein the most obvious function of which is the
      down-regulation of the growth suppressor protein p53. It represents a highly
      phosphorylated protein but only little is yet known about the sites
      phosphorylated in vivo, the kinases that are responsible for the phosphorylation 
      or the functional relevance of the phosphorylation status. Recently, we have
      shown that mdm2 is a good substrate for protein kinase CK2 at least in vitro.
      Computer analysis of the primary amino acid sequence of mdm2 revealed 19 putative
      CK2 phosphorylation sites. By using deletion mutants of mdm2 and a peptide
      library we identified the serine residue at position 269 which lies within a
      canonical CK2 consensus sequence (EGQELSDEDDE) as the most important CK2
      phosphorylation site. Moreover, by using the mdm2 S269A mutant for in vitro
      phosphorylation assays this site was shown to be phosphorylated by CK2. Binding
      studies revealed that phosphorylation of mdm2 at S269 does not have any influence
      on the binding of p53 to mdm2.
FAU - Gotz, C
AU  - Gotz C
AD  - Department of Medical Biochemistry and Molecular Biology, University of the
      Saarland, Homburg, Germany. bccgoe@med-rz-uni-sb.de
FAU - Kartarius, S
AU  - Kartarius S
FAU - Scholtes, P
AU  - Scholtes P
FAU - Nastainczyk, W
AU  - Nastainczyk W
FAU - Montenarh, M
AU  - Montenarh M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Codon)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptides)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 2.3.2.27 (MDM2 protein, human)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Binding Sites
MH  - Blotting, Western
MH  - Casein Kinase II
MH  - Cell Line
MH  - Codon
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/metabolism
MH  - Humans
MH  - Insecta
MH  - Molecular Sequence Data
MH  - Mutation
MH  - *Nuclear Proteins
MH  - Peptides/chemistry
MH  - Phosphorylation
MH  - Plasmids/metabolism
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Protein-Serine-Threonine Kinases/*chemistry
MH  - Proto-Oncogene Proteins/*chemistry/metabolism
MH  - Proto-Oncogene Proteins c-mdm2
MH  - Recombinant Proteins/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Tumor Suppressor Protein p53/metabolism
EDAT- 1999/11/24 09:00
MHDA- 2002/12/04 04:00
CRDT- 1999/11/24 09:00
PHST- 1999/11/24 09:00 [pubmed]
PHST- 2002/12/04 04:00 [medline]
PHST- 1999/11/24 09:00 [entrez]
AID - ejb882 [pii]
AID - 10.1046/j.1432-1327.1999.00882.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Dec;266(2):493-501. doi: 10.1046/j.1432-1327.1999.00882.x.