PMID- 10561575
OWN - NLM
STAT- MEDLINE
DCOM- 20021203
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 266
IP  - 2
DP  - 1999 Dec
TI  - Characterization and analysis of a novel glycoprotein from snake venom using
      liquid chromatography-electrospray mass spectrometry and Edman degradation.
PG  - 352-8
AB  - An N-linked glycosylation in a novel C-lectin protein from snake venom was
      observed by Edman degradation and liquid chromatography-electrospray mass
      spectrometry. The peptides obtained by trypsin cleavage were analyzed to confirm 
      the amino acid sequence and Asn5 was found to be the N-glycosylation site. The
      result was further confirmed by N-glycosidase digestion. In addition, the protein
      and tryptic peptides with and without glycan chain were characterized by mass
      spectrometry according to the mass difference. The glycopeptide obtained from
      proteolytic digestion was analyzed and the glycoforms were identified as
      high-mannose type by tandem MS coupled with alpha-mannosidase digestion. An
      oxidized Met residue was detected and located in the protein by mass
      spectrometry.
FAU - Zeng, R
AU  - Zeng R
AD  - Shanghai Institute of Biochemistry, Chinese Academy of Sciences, China.
FAU - Xu, Q
AU  - Xu Q
FAU - Shao, X X
AU  - Shao XX
FAU - Wang, K Y
AU  - Wang KY
FAU - Xia, Q C
AU  - Xia QC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Amino Acids)
RN  - 0 (Glycoproteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Snake Venoms)
RN  - 0 (Trimeresurus stejnegeri lectin)
RN  - 0 (Viper Venoms)
RN  - EC 3.2.1.- (Mannosidases)
RN  - EC 3.2.1.24 (alpha-Mannosidase)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.5.- (Amidohydrolases)
RN  - EC 3.5.1.52 (Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase)
SB  - IM
MH  - Amidohydrolases/metabolism
MH  - Amino Acids/chemistry
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid/*methods
MH  - Glycoproteins/chemistry/*isolation & purification
MH  - Glycosylation
MH  - Lectins, C-Type/*chemistry/*isolation & purification
MH  - Mannosidases/pharmacology
MH  - Mass Spectrometry
MH  - Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
MH  - Protein Structure, Tertiary
MH  - Snake Venoms/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Time Factors
MH  - Trimeresurus/metabolism
MH  - Trypsin/pharmacology
MH  - Viper Venoms/*chemistry/*isolation & purification
MH  - alpha-Mannosidase
EDAT- 1999/11/24 09:00
MHDA- 2002/12/04 04:00
CRDT- 1999/11/24 09:00
PHST- 1999/11/24 09:00 [pubmed]
PHST- 2002/12/04 04:00 [medline]
PHST- 1999/11/24 09:00 [entrez]
AID - ejb859 [pii]
AID - 10.1046/j.1432-1327.1999.00859.x [doi]
PST - ppublish
SO  - Eur J Biochem. 1999 Dec;266(2):352-8. doi: 10.1046/j.1432-1327.1999.00859.x.