PMID- 10561551
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1435
IP  - 1-2
DP  - 1999 Nov 16
TI  - Molecular cloning and characterization of a new fasting-inducible short-chain
      dehydrogenase/reductase from rat liver(1).
PG  - 184-90
AB  - We have isolated a 668 bp cDNA from fasted rat liver, designated RLF98, by
      suppression subtractive hybridization (SSH). The full-length RLF98 cDNA, cloned
      by rapid amplification of cDNA ends (RACE), is 1113 bp long with an open reading 
      frame of 912 bp. This cDNA encodes a protein of 303 amino acid residues with a
      calculated molecular weight of 32433 Da. In vitro transcription and translation
      of the full-length RLF98 cDNA produced a protein of about 33 kDa. The RLF98
      protein shares strong amino acid sequence homology with members of the
      short-chain dehydrogenase/reductase (SDR) family. Northern analysis of RNA from
      rat liver revealed a transcript of 1.1 kb. Fasting increased this mRNA 2.7-fold. 
      While the RLF98 gene is widely expressed in rat tissues, its level of expression 
      is highly variable. Expression in liver and kidney is abundant and is more than
      10 times that observed in other tissues. Our data indicate that the RLF98 is a
      new member of the SDR family that is upregulated by fasting. Additional
      experiments including purification of recombinant RLF98 protein are in progress
      to define the specific function of this protein and the role it plays during
      fasting-induced catabolism.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of Pediatrics, 509 Burnett-Womack Building, CB# 7220, The University
      of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
FAU - Underwood, L E
AU  - Underwood LE
LA  - eng
SI  - GENBANK/AF099742
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA, Complementary)
RN  - 488-81-3 (Ribitol)
RN  - 63231-63-0 (RNA)
RN  - EC 1.1.1.1 (Alcohol Dehydrogenase)
RN  - EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors)
RN  - EC 1.3.1.38 (trans-2-enoyl-CoA reductase (NADPH))
SB  - IM
MH  - Alcohol Dehydrogenase/chemistry/*genetics/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry/isolation & purification
MH  - Fasting
MH  - Liver/*enzymology
MH  - Molecular Sequence Data
MH  - Oxidoreductases Acting on CH-CH Group Donors
MH  - Protein Biosynthesis
MH  - RNA/chemistry/isolation & purification
MH  - Rats
MH  - Ribitol/metabolism
MH  - Transcription, Genetic
MH  - Transfection
MH  - Up-Regulation
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - S0167483899002186 [pii]
AID - 10.1016/s0167-4838(99)00218-6 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1999 Nov 16;1435(1-2):184-90. doi:
      10.1016/s0167-4838(99)00218-6.