PMID- 10559986
OWN - NLM
STAT- MEDLINE
DCOM- 20010419
LR  - 20191210
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 1
IP  - 7
DP  - 1999 Nov
TI  - Evidence for a COP-I-independent transport route from the Golgi complex to the
      endoplasmic reticulum.
PG  - 423-30
AB  - The cytosolic coat-protein complex COP-I interacts with cytoplasmic 'retrieval'
      signals present in membrane proteins that cycle between the endoplasmic reticulum
      (ER) and the Golgi complex, and is required for both anterograde and retrograde
      transport in the secretory pathway. Here we study the role of COP-I in
      Golgi-to-ER transport of several distinct marker molecules. Microinjection of
      anti-COP-I antibodies inhibits retrieval of the lectin-like molecule ERGIC-53 and
      of the KDEL receptor from the Golgi to the ER. Transport to the ER of protein
      toxins, which contain a sequence that is recognized by the KDEL receptor, is also
      inhibited. In contrast, microinjection of anti-COP-I antibodies or expression of 
      a GTP-restricted Arf-1 mutant does not interfere with Golgi-to-ER transport of
      Shiga toxin/Shiga-like toxin-1 or with the apparent recycling to the ER of
      Golgi-resident glycosylation enzymes. Overexpression of a GDP-restricted mutant
      of Rab6 blocks transport to the ER of Shiga toxin/Shiga-like toxin-1 and
      glycosylation enzymes, but not of ERGIC-53, the KDEL receptor or KDEL-containing 
      toxins. These data indicate the existence of at least two distinct pathways for
      Golgi-to-ER transport, one COP-I dependent and the other COP-I independent. The
      COP-I-independent pathway is specifically regulated by Rab6 and is used by Golgi 
      glycosylation enzymes and Shiga toxin/Shiga-like toxin-1.
FAU - Girod, A
AU  - Girod A
AD  - Cell Biology and Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 
      Heidelberg, Germany.
FAU - Storrie, B
AU  - Storrie B
FAU - Simpson, J C
AU  - Simpson JC
FAU - Johannes, L
AU  - Johannes L
FAU - Goud, B
AU  - Goud B
FAU - Roberts, L M
AU  - Roberts LM
FAU - Lord, J M
AU  - Lord JM
FAU - Nilsson, T
AU  - Nilsson T
FAU - Pepperkok, R
AU  - Pepperkok R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Coat Protein Complex I)
RN  - 0 (KDEL receptor)
RN  - 0 (LMAN1 protein, human)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Protein Sorting Signals)
RN  - 0 (Rab6 protein)
RN  - 0 (Receptors, Peptide)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Shiga Toxin 1)
RN  - 0 (Vesicular Transport Proteins)
RN  - 75757-64-1 (Shiga Toxin)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factor 1)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
RN  - EC 3.6.5.2 (SAR1 protein, S cerevisiae)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - ADP-Ribosylation Factor 1/genetics/metabolism
MH  - Amino Acid Motifs
MH  - Animals
MH  - Chlorocebus aethiops
MH  - Coat Protein Complex I/*metabolism
MH  - Endoplasmic Reticulum/*metabolism
MH  - Golgi Apparatus/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - *Mannose-Binding Lectins
MH  - Membrane Proteins/metabolism
MH  - Microinjections
MH  - Microscopy, Fluorescence
MH  - Monomeric GTP-Binding Proteins/genetics/metabolism
MH  - Protein Sorting Signals
MH  - Protein Transport/*physiology
MH  - Receptors, Peptide/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Shiga Toxin/*metabolism
MH  - Shiga Toxin 1/*metabolism
MH  - Vero Cells
MH  - Vesicular Transport Proteins
MH  - rab GTP-Binding Proteins/metabolism
EDAT- 1999/11/24 09:00
MHDA- 2001/04/21 10:01
CRDT- 1999/11/24 09:00
PHST- 1999/11/24 09:00 [pubmed]
PHST- 2001/04/21 10:01 [medline]
PHST- 1999/11/24 09:00 [entrez]
AID - 10.1038/15658 [doi]
PST - ppublish
SO  - Nat Cell Biol. 1999 Nov;1(7):423-30. doi: 10.1038/15658.