PMID- 10559961
OWN - NLM
STAT- MEDLINE
DCOM- 20000908
LR  - 20090610
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 1
IP  - 6
DP  - 1999 Oct
TI  - Formation of AP-3 transport intermediates requires Vps41 function.
PG  - 346-53
AB  - Transport of a subset of membrane proteins to the yeast vacuole requires the
      function of the AP-3 adaptor protein complex. To define the molecular
      requirements of vesicular transport in this pathway, we used a biochemical
      approach to analyse the formation and content of the AP-3 transport intermediate.
      A vam3tsf (vacuolar t-SNARE) mutant blocks vesicle docking and fusion with the
      vacuole and causes the accumulation of 50-130-nanometre membrane vesicles, which 
      we isolated and showed by biochemical analysis and immunocytochemistry to contain
      both AP-3 adaptors and alkaline phosphatase (ALP) pathway cargoes. Inactivation
      of AP-3 or the protein Vps41 blocks formation of this vesicular intermediate.
      Vps41 binds to the AP-3 delta-adaptin subunit, suggesting that they function
      together in the formation of ALP pathway transport intermediates at the late
      Golgi.
FAU - Rehling, P
AU  - Rehling P
AD  - Division of Cellular and Molecular Medicine and Howard Hughes Medical Institute, 
      University of California, San Diego, La Jolla, California 92093-0668, USA.
FAU - Darsow, T
AU  - Darsow T
FAU - Katzmann, D J
AU  - Katzmann DJ
FAU - Emr, S D
AU  - Emr SD
LA  - eng
GR  - CA58689/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Adaptor Protein Complex alpha Subunits)
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Carrier Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Monomeric Clathrin Assembly Proteins)
RN  - 0 (NPL3 protein, S cerevisiae)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Qa-SNARE Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (VAM3 protein, S cerevisiae)
RN  - 0 (VPS41 protein, S cerevisiae)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (clathrin assembly protein AP180)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Adaptor Protein Complex alpha Subunits
MH  - Adaptor Proteins, Vesicular Transport
MH  - Alkaline Phosphatase/genetics/metabolism
MH  - Binding Sites
MH  - Biological Transport
MH  - Carrier Proteins/genetics/*metabolism/physiology
MH  - Cell Compartmentation
MH  - Cell Membrane/metabolism
MH  - Fungal Proteins/genetics/*metabolism/physiology
MH  - Membrane Proteins/genetics/*metabolism
MH  - *Monomeric Clathrin Assembly Proteins
MH  - Mutagenesis
MH  - *Nuclear Proteins
MH  - Qa-SNARE Proteins
MH  - RNA-Binding Proteins/genetics/*metabolism/physiology
MH  - *Saccharomyces cerevisiae Proteins
MH  - Vacuoles/metabolism
MH  - *Vesicular Transport Proteins
EDAT- 1999/11/24 09:00
MHDA- 2000/09/19 11:01
CRDT- 1999/11/24 09:00
PHST- 1999/11/24 09:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 1999/11/24 09:00 [entrez]
AID - 10.1038/14037 [doi]
PST - ppublish
SO  - Nat Cell Biol. 1999 Oct;1(6):346-53. doi: 10.1038/14037.