PMID- 10559958
OWN - NLM
STAT- MEDLINE
DCOM- 20000908
LR  - 20091119
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 1
IP  - 6
DP  - 1999 Oct
TI  - The lectin ERGIC-53 is a cargo transport receptor for glycoproteins.
PG  - 330-4
AB  - Soluble secretory proteins are transported from the endoplasmic reticulum (ER) to
      the ER-Golgi intermediate compartment (ERGIC) in vesicles coated with COP-II coat
      proteins. The sorting of secretory cargo into these vesicles is thought to
      involve transmembrane cargo-receptor proteins. Here we show that a
      cathepsin-Z-related glycoprotein binds to the recycling, mannose-specific
      membrane lectin ERGIC-53. Binding occurs in the ER, is carbohydrate- and
      calcium-ion-dependent and is affected by untrimmed glucose residues. Binding does
      not, however, require oligomerization of ERGIC-53, although oligomerization is
      required for exit of ERGIC-53 from the ER. Dissociation of ERGIC-53 occurs in the
      ERGIC and is delayed if ERGIC-53 is mislocalized to the ER. These results
      strongly indicate that ERGIC-53 may function as a receptor facilitating
      ER-to-ERGIC transport of soluble glycoprotein cargo.
FAU - Appenzeller, C
AU  - Appenzeller C
AD  - Department of Pharmacology/Neurobiology, University of Basel, Klingelbergstrasse 
      70, CH-4056 Basel, Switzerland.
FAU - Andersson, H
AU  - Andersson H
FAU - Kappeler, F
AU  - Kappeler F
FAU - Hauri, H P
AU  - Hauri HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Carrier Proteins)
RN  - 0 (ERGIC-53 protein, mouse)
RN  - 0 (Glycoproteins)
RN  - 0 (LMAN1 protein, human)
RN  - 0 (Lectins)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Membrane Proteins)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.18.1 (CTSZ protein, human)
RN  - EC 3.4.18.1 (Cathepsin Z)
RN  - EC 3.4.18.1 (Ctsz protein, mouse)
RN  - EC 3.4.22.38 (CTSK protein, human)
RN  - EC 3.4.22.38 (Cathepsin K)
RN  - EC 3.4.22.38 (Ctsk protein, mouse)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carbohydrate Metabolism
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cathepsin K
MH  - Cathepsin Z
MH  - Cathepsins/*metabolism
MH  - Cell Line
MH  - Endoplasmic Reticulum/*metabolism
MH  - Glycoproteins/*metabolism
MH  - Humans
MH  - Lectins/genetics/*metabolism
MH  - *Mannose-Binding Lectins
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Rabbits
EDAT- 1999/11/24 09:00
MHDA- 2000/09/19 11:01
CRDT- 1999/11/24 09:00
PHST- 1999/11/24 09:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 1999/11/24 09:00 [entrez]
AID - 10.1038/14020 [doi]
PST - ppublish
SO  - Nat Cell Biol. 1999 Oct;1(6):330-4. doi: 10.1038/14020.