PMID- 10559899
OWN - NLM
STAT- MEDLINE
DCOM- 19991216
LR  - 20071115
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 1
IP  - 3
DP  - 1999 Jul
TI  - Cooperation between mDia1 and ROCK in Rho-induced actin reorganization.
PG  - 136-43
AB  - The small GTPase Rho induces the formation of actin stress fibres and mediates
      the formation of diverse actin structures. However, it remains unclear how Rho
      regulates its effectors to elicit such functions. Here we show that GTP-bound Rho
      activates its effector mDia1 by disrupting mDia1's intramolecular interactions.
      Active mDia1 induces the formation of thin actin stress fibres, which are
      disorganized in the absence of activity of the Rho-associated kinase ROCK.
      Moreover, active mDia1 transforms ROCK-induced condensed actin fibres into
      structures reminiscent of Rho-induced stress fibres. Thus mDia1 and ROCK work
      concurrently during Rho-induced stress-fibre formation. Intriguingly, mDia1 and
      ROCK, depending on the balance of the two activities, induce actin fibres of
      various thicknesses and densities. Thus Rho may induce the formation of different
      actin structures affected by the balance between mDia1 and ROCK signalling.
FAU - Watanabe, N
AU  - Watanabe N
AD  - Department of Pharmacology, Kyoto University Faculty of Medicine, Japan.
FAU - Kato, T
AU  - Kato T
FAU - Fujita, A
AU  - Fujita A
FAU - Ishizaki, T
AU  - Ishizaki T
FAU - Narumiya, S
AU  - Narumiya S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
SB  - IM
CIN - Nat Cell Biol. 1999 Jul;1(3):E64-6. PMID: 10559912
MH  - Actins/*chemistry/*metabolism/ultrastructure
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Guanosine Triphosphate/metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Models, Chemical
MH  - Mutagenesis, Site-Directed
MH  - Protein-Serine-Threonine Kinases/*metabolism
MH  - Recombinant Proteins/chemistry/metabolism/ultrastructure
MH  - Sequence Deletion
MH  - Signal Transduction
MH  - Transfection
MH  - rho GTP-Binding Proteins/*metabolism
MH  - rho-Associated Kinases
EDAT- 1999/11/13 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/11/13 09:00
PHST- 1999/11/13 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/11/13 09:00 [entrez]
AID - 10.1038/11056 [doi]
PST - ppublish
SO  - Nat Cell Biol. 1999 Jul;1(3):136-43. doi: 10.1038/11056.