PMID- 10559856
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20161124
IS  - 1465-7392 (Print)
IS  - 1465-7392 (Linking)
VI  - 1
IP  - 1
DP  - 1999 May
TI  - Spatial control of coated-pit dynamics in living cells.
PG  - 1-7
AB  - Here we visualize new aspects of the dynamics of endocytotic clathrin-coated pits
      and vesicles in mammalian cells by using a fusion protein consisting of green
      fluorescent protein and clathrin light chain a. Clathrin-coated pits invaginating
      from the plasma membrane show definite, but highly limited, mobility within the
      membrane that is relaxed upon treatment with latrunculin B, an inhibitor of actin
      assembly, indicating that an actin-based framework may be involved in the
      mobility of these pits. Transient, motile coated vesicles that originate from
      coated pits can be detected, with multiple vesicles occasionally appearing to
      emanate from a single pit. Despite their seemingly random distribution, coated
      pits tend to form repeatedly at defined sites while excluding other regions. This
      spatial regulation of coated-pit assembly and function is attributable to the
      attachment of the coated pits to the membrane skeleton.
FAU - Gaidarov, I
AU  - Gaidarov I
AD  - Kimmel Cancer Institute, Philadelphia, Pennsylvania, USA.
FAU - Santini, F
AU  - Santini F
FAU - Warren, R A
AU  - Warren RA
FAU - Keen, J H
AU  - Keen JH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nat Cell Biol
JT  - Nature cell biology
JID - 100890575
RN  - 0 (Bridged Bicyclo Compounds, Heterocyclic)
RN  - 0 (Clathrin)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Marine Toxins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Thiazoles)
RN  - 0 (Thiazolidines)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - LW7U308U7U (latrunculin B)
SB  - IM
MH  - Animals
MH  - Bridged Bicyclo Compounds, Heterocyclic/pharmacology
MH  - COS Cells
MH  - Cell Membrane/drug effects/physiology
MH  - Clathrin/*metabolism
MH  - Coated Pits, Cell-Membrane/drug effects/*physiology/*ultrastructure
MH  - Endocytosis
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Green Fluorescent Proteins
MH  - Luminescent Proteins/metabolism
MH  - Marine Toxins/pharmacology
MH  - Mice
MH  - Placenta/metabolism
MH  - Pregnancy
MH  - Recombinant Fusion Proteins/metabolism
MH  - Thiazoles/pharmacology
MH  - Thiazolidines
EDAT- 1999/11/13 09:00
MHDA- 2001/03/23 10:01
CRDT- 1999/11/13 09:00
PHST- 1999/11/13 09:00 [pubmed]
PHST- 2001/03/23 10:01 [medline]
PHST- 1999/11/13 09:00 [entrez]
AID - 10.1038/8971 [doi]
PST - ppublish
SO  - Nat Cell Biol. 1999 May;1(1):1-7. doi: 10.1038/8971.