PMID- 10559782
OWN - NLM
STAT- MEDLINE
DCOM- 19991215
LR  - 20171116
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 28
IP  - 3
DP  - 1999 Dec
TI  - Evidence for the presence of N-CAM 180 on astrocytes from rat cerebellum and
      differences in glycan structures between N-CAM 120 and N-CAM 140.
PG  - 236-43
AB  - Differences in the N-linked oligosaccharides on isoforms of the neural cell
      adhesion molecule (N-CAM) on astrocytes were found using a lectin, namely, Datura
      stramonium agglutinin (DSA). Integral proteins of astrocytes prepared from
      newborn rat cerebella were solubilized with Nonident P-40 and then separated into
      two fractions, an unbound fraction and a bound fraction, by a DSA-agarose column.
      Both fractions thus obtained were subjected to immunoblotting using an anti-N-CAM
      monoclonal antibody. In the DSA-bound fraction, N-CAM 180 was found as well as
      N-CAM 120 and N-CAM 140. On the other hand, N-CAM 180 was not detected in the
      unbound fraction, whereas N-CAM 120 and N-CAM 140 were. Furthermore, N-CAM 180
      did not carry the HNK-1 epitope, whereas the other two isoforms did. Although the
      presence of N-CAM 180 on astrocytes was controversial until recently, the results
      shown here indicate that N-CAM 180 exists on rat astrocytes and exclusively
      carries a glycan structure reacting with DSA. This is the first demonstration of 
      the production of N-CAM glycoforms carrying different oligosaccharides by a
      homogeneous astrocyte preparation. These results suggest that the glycosylation
      of each N-CAM isoform might be regulated independently. Whether each N-CAM with
      different glycans participates in different functions remains to be established.
CI  - Copyright 1999 Wiley-Liss, Inc.
FAU - Sasaki, T
AU  - Sasaki T
AD  - Department of Glycobiology, Tokyo Metropolitan Institute of Gerontology,
      Sakaecho, Itabashi-ku, Tokyo, Japan.
FAU - Endo, T
AU  - Endo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (CD57 Antigens)
RN  - 0 (Datura stramonium lectin)
RN  - 0 (Lectins)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Plant Lectins)
RN  - 0 (Polysaccharides)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - CD57 Antigens/analysis
MH  - Cerebellum/*metabolism
MH  - Electrophoresis, Agar Gel
MH  - Humans
MH  - Immunoblotting
MH  - Lectins
MH  - Neural Cell Adhesion Molecules/*chemistry/*metabolism
MH  - *Plant Lectins
MH  - Polysaccharides/*chemistry
MH  - Precipitin Tests
MH  - Protein Isoforms/chemistry/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Solubility
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1002/(SICI)1098-1136(199912)28:3<236::AID-GLIA7>3.0.CO;2-# [pii]
PST - ppublish
SO  - Glia. 1999 Dec;28(3):236-43.