PMID- 10559261
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - Reconstitution of proliferating cell nuclear antigen-dependent repair of
      apurinic/apyrimidinic sites with purified human proteins.
PG  - 33703-8
AB  - An apurinic/apyrimidinic (AP) site is one of the most abundant lesions
      spontaneously generated in living cells and is also a reaction intermediate in
      base excision repair. In higher eukaryotes, there are two alternative pathways
      for base excision repair: a DNA polymerase beta-dependent pathway and a
      proliferating cell nuclear antigen (PCNA)-dependent pathway. Here we have
      reconstituted PCNA-dependent repair of AP sites with six purified human proteins:
      AP endonuclease, replication factor C, PCNA, flap endonuclease 1 (FEN1), DNA
      polymerase delta, and DNA ligase I. The length of nucleotides replaced during the
      repair reaction (patch size) was predominantly two nucleotides, although longer
      patches of up to seven nucleotides could be detected. Neither replication protein
      A nor Ku70/80 enhanced the repair activity in this system. Disruption of the
      PCNA-binding site of either FEN1 or DNA ligase I significantly reduced efficiency
      of AP site repair but did not affect repair patch size.
FAU - Matsumoto, Y
AU  - Matsumoto Y
AD  - Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia,
      Pennsylvania 19111, USA. y_matsumoto@fccc.edu
FAU - Kim, K
AU  - Kim K
FAU - Hurwitz, J
AU  - Hurwitz J
FAU - Gary, R
AU  - Gary R
FAU - Levin, D S
AU  - Levin DS
FAU - Tomkinson, A E
AU  - Tomkinson AE
FAU - Park, M S
AU  - Park MS
LA  - eng
GR  - CA06927/CA/NCI NIH HHS/United States
GR  - CA63154/CA/NCI NIH HHS/United States
GR  - CA71630/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (LIG1 protein, human)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Proteins)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.- (Flap Endonucleases)
RN  - EC 3.1.11.- (FEN1 protein, human)
RN  - EC 3.1.11.5 (Exodeoxyribonuclease V)
RN  - EC 6.5.1.- (DNA Ligases)
RN  - EC 6.5.1.1 (DNA Ligase ATP)
SB  - IM
MH  - Base Sequence
MH  - DNA Ligase ATP
MH  - DNA Ligases/genetics/metabolism
MH  - DNA Primers
MH  - *DNA Repair
MH  - Exodeoxyribonuclease V
MH  - Exodeoxyribonucleases/genetics/metabolism
MH  - *Flap Endonucleases
MH  - Humans
MH  - Proliferating Cell Nuclear Antigen/*metabolism
MH  - Proteins/genetics/*metabolism
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33703 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33703-8. doi: 10.1074/jbc.274.47.33703.