PMID- 10559260
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - Long patch base excision repair with purified human proteins. DNA ligase I as
      patch size mediator for DNA polymerases delta and epsilon.
PG  - 33696-702
AB  - Among the different base excision repair pathways known, the long patch base
      excision repair of apurinic/apyrimidinic sites is an important mechanism that
      requires proliferating cell nuclear antigen. We have reconstituted this pathway
      using purified human proteins. Our data indicated that efficient repair is
      dependent on six components including AP endonuclease, replication factor C,
      proliferating cell nuclear antigen, DNA polymerases delta or epsilon, flap
      endonuclease 1, and DNA ligase I. Fine mapping of the nucleotide replacement
      events showed that repair patches extended up to a maximum of 10 nucleotides 3'
      to the lesion. However, almost 70% of the repair synthesis was confined to
      2-4-nucleotide patches and DNA ligase I appeared to be responsible for limiting
      the repair patch length. Moreover, both proliferating cell nuclear antigen and
      flap endonuclease 1 are required for the production and ligation of long patch
      repair intermediates suggesting an important role of this complex in both
      excision and resynthesis steps.
FAU - Pascucci, B
AU  - Pascucci B
AD  - Laboratory of Comparative Toxicology, Istituto Superiore di Sanita, Viale Regina 
      Elena 299, 00161 Rome, Italy.
FAU - Stucki, M
AU  - Stucki M
FAU - Jonsson, Z O
AU  - Jonsson ZO
FAU - Dogliotti, E
AU  - Dogliotti E
FAU - Hubscher, U
AU  - Hubscher U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (LIG1 protein, human)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - EC 2.7.7.- (DNA Polymerase II)
RN  - EC 2.7.7.- (DNA Polymerase III)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.- (Flap Endonucleases)
RN  - EC 3.1.11.- (FEN1 protein, human)
RN  - EC 3.1.11.5 (Exodeoxyribonuclease V)
RN  - EC 3.1.21.2 (Deoxyribonuclease IV (Phage T4-Induced))
RN  - EC 4.2.- (Carbon-Oxygen Lyases)
RN  - EC 4.2.99.18 (DNA-(Apurinic or Apyrimidinic Site) Lyase)
RN  - EC 6.5.1.- (DNA Ligases)
RN  - EC 6.5.1.1 (DNA Ligase ATP)
SB  - IM
MH  - Base Sequence
MH  - Carbon-Oxygen Lyases/metabolism
MH  - DNA Ligase ATP
MH  - DNA Ligases/*metabolism
MH  - DNA Polymerase II/*metabolism
MH  - DNA Polymerase III/*metabolism
MH  - DNA Primers
MH  - *DNA Repair
MH  - DNA-(Apurinic or Apyrimidinic Site) Lyase
MH  - Deoxyribonuclease IV (Phage T4-Induced)
MH  - Exodeoxyribonuclease V
MH  - Exodeoxyribonucleases/metabolism
MH  - *Flap Endonucleases
MH  - Humans
MH  - Proliferating Cell Nuclear Antigen/metabolism
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33696 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33696-702. doi: 10.1074/jbc.274.47.33696.