PMID- 10559254
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - The kinesin-like motor protein KIF1C occurs in intact cells as a dimer and
      associates with proteins of the 14-3-3 family.
PG  - 33654-60
AB  - Proteins of the kinesin superfamily are regulated in their motor activity as well
      as in their ability to bind to their cargo by carboxyl-terminal associating
      proteins and phosphorylation. KIF1C, a recently identified member of the
      KIF1/Unc104 family, was shown to be involved in the retrograde vesicle transport 
      from the Golgi-apparatus to the endoplasmic reticulum. In a yeast two-hybrid
      screen using the carboxyl-terminal 350 amino acids of KIF1C as a bait, we
      identified as binding proteins 14-3-3 beta, gamma, epsilon, and zeta. In
      addition, a clone encoding the carboxyl-terminal 290 amino acids of KIF1C was
      found, indicating a potential for KIF1C to dimerize. Subsequent transient
      overexpression experiments showed that KIF1C can dimerize efficiently. However,
      in untransfected cells, only a small portion of KIF1C was detected as a dimer.
      The association of 14-3-3 proteins with KIF1C could be confirmed in transient
      expression systems and in untransfected cells and was dependent on the
      phosphorylation of serine 1092 located in a consensus binding sequence for 14-3-3
      ligands. Serine 1092 was a substrate for the protein kinase casein kinase II in
      vitro, and inhibition of casein kinase II in cells diminished the association of 
      KIF1C with 14-3-3gamma. Our data thus suggest that KIF1C can form dimers and is
      associated with proteins of the 14-3-3 family.
FAU - Dorner, C
AU  - Dorner C
AD  - Medical Clinic IV, University of Tubingen, Tubingen, 72076 Germany.
FAU - Ullrich, A
AU  - Ullrich A
FAU - Haring, H U
AU  - Haring HU
FAU - Lammers, R
AU  - Lammers R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (14-3-3 Proteins)
RN  - 0 (KIF1C protein, human)
RN  - 0 (Kif1c protein, mouse)
RN  - 0 (Proteins)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 3.6.4.4 (Kinesin)
SB  - IM
MH  - 14-3-3 Proteins
MH  - Animals
MH  - Binding Sites
MH  - Casein Kinase II
MH  - Cell Line
MH  - Dimerization
MH  - Humans
MH  - Kinesin/chemistry/*metabolism
MH  - Mice
MH  - Phosphorylation
MH  - Protein Binding
MH  - Protein-Serine-Threonine Kinases/metabolism
MH  - Proteins/*metabolism
MH  - Substrate Specificity
MH  - Two-Hybrid System Techniques
MH  - *Tyrosine 3-Monooxygenase
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33654 [doi]
AID - S0021-9258(17)46512-4 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33654-60. doi: 10.1074/jbc.274.47.33654.