PMID- 10559246
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - Identification and characterization of hPEM-2, a guanine nucleotide exchange
      factor specific for Cdc42.
PG  - 33587-93
AB  - Guanine nucleotide exchange factors of the Dbl family regulate the actin
      cytoskeleton through activation of Rho-like GTPases. At present the Dbl family
      consists of more than thirty members; many have not been phenotypically or
      biochemically characterized. Guanine nucleotide exchange factors universally
      feature a Dbl homology domain followed by a pleckstrin homology domain. Employing
      data base screening we identified a recently cloned cDNA, KIAA0424, showing
      substantial sequence homology with Rac activators such as Tiam1, Sos, Vav, and
      PIX within the catalytic domain. This cDNA appears to be the human homologue of
      the Ascidian protein Posterior End Mark-2 (PEM-2). We refer to this exchanger as 
      hPEM-2. hPEM-2 encodes a protein of 70 kDa and features an N-terminal src
      homology 3 domain, followed by tandem Dbl homology and pleckstrin homology
      domains. The gene is highly expressed in brain and is localized on the human
      X-chromosome. Employing biochemical activity assays for Rho-like GTPases we found
      that hPEM-2 specifically activates Cdc42 and not Rac or RhoA. Ectopic expression 
      of hPEM-2 in NIH3T3 fibroblasts revealed a Cdc42 phenotype featuring filopodia
      formation, followed by cortical actin polymerization and cell rounding. hPEM-2
      represents an exchange factor, which may have a role in the regulation of a
      number of cellular processes through Cdc42.
FAU - Reid, T
AU  - Reid T
AD  - Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066
      CX Amsterdam, The Netherlands.
FAU - Bathoorn, A
AU  - Bathoorn A
FAU - Ahmadian, M R
AU  - Ahmadian MR
FAU - Collard, J G
AU  - Collard JG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (Egg Proteins)
RN  - 0 (Guanine Nucleotide Exchange Factors)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PEM-2 protein, Ciona savignyi)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - DNA Primers
MH  - Egg Proteins/chemistry/*metabolism
MH  - Enzyme Activation
MH  - GTP Phosphohydrolases/metabolism
MH  - Guanine Nucleotide Exchange Factors/chemistry/*metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/*metabolism
MH  - Phenotype
MH  - Protein Binding
MH  - Sequence Homology, Amino Acid
MH  - cdc42 GTP-Binding Protein/*metabolism
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33587 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33587-93. doi: 10.1074/jbc.274.47.33587.