PMID- 10559239
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20211203
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - Activation of rat frizzled-1 promotes Wnt signaling and differentiation of mouse 
      F9 teratocarcinoma cells via pathways that require Galpha(q) and Galpha(o)
      function.
PG  - 33539-44
AB  - The frizzled gene family of putative Wnt receptors encodes proteins that have a
      seven transmembrane-spanning motif characteristic of G-protein-linked receptors, 
      although no loss-of-function studies have demonstrated a requirement for
      G-proteins for Wnt signaling by the gene product of frizzled-1. Medium
      conditioned by mouse F9 teratocarcinoma stem cells stably transfected to express 
      either Xenopus Wnt-5a or Wnt-8 was used to test primitive endoderm formation of
      F9 stem cells. F9 stem cells expressing the rat Frizzled-1 receptors demonstrated
      endoderm formation in response to conditioned medium containing Wnt-8 but not to 
      medium containing Wnt-5a. Primitive endoderm formation stimulated by Wnt-8 acting
      on the rat Frizzled-1 receptor was blocked by treatment with pertussis toxin by
      depletion of either Galpha(o) or Galpha(q) via antisense oligodeoxynucleotides,
      as well as by inhibitors of protein kinase C (bisindoylmaleimide) and of
      mitogen-activated protein kinase kinase (PD98059). Our results demonstrate the
      requirement for G-protein subunits Galpha(o) (a pertussis toxin substrate) and
      Galpha(q) for signaling by Frizzled-1, and an obligate role for the protein
      kinase C (likely mediated through stimulation of Galpha(q)) and mitogen-activated
      protein kinase network at the level of mitogen-activated protein kinase kinase.
FAU - Liu, T
AU  - Liu T
AD  - Department of Pharmacology, State University of New York, Stony Brook, New York
      11794-8651, USA.
FAU - Liu, X
AU  - Liu X
FAU - Wang, H y
AU  - Wang Hy
FAU - Moon, R T
AU  - Moon RT
FAU - Malbon, C C
AU  - Malbon CC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (FZD1 protein, Xenopus)
RN  - 0 (Frizzled Receptors)
RN  - 0 (Fzd1 protein, mouse)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Receptors, Neurotransmitter)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 0 (Wnt Proteins)
RN  - 0 (Xenopus Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 149748-09-4 (Fzd1 protein, rat)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 1)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (Map3k1 protein, mouse)
RN  - EC 3.1.4.35 (3',5'-Cyclic-GMP Phosphodiesterases)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
MH  - 3',5'-Cyclic-GMP Phosphodiesterases/antagonists & inhibitors
MH  - Animals
MH  - Clone Cells
MH  - Culture Media, Conditioned
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Frizzled Receptors
MH  - GTP-Binding Proteins/antagonists & inhibitors/*metabolism
MH  - *MAP Kinase Kinase Kinase 1
MH  - MAP Kinase Kinase Kinases/antagonists & inhibitors
MH  - Mice
MH  - Pertussis Toxin
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Kinase C/antagonists & inhibitors
MH  - *Protein Serine-Threonine Kinases
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Rats
MH  - Receptors, G-Protein-Coupled
MH  - Receptors, Neurotransmitter/*metabolism
MH  - *Signal Transduction
MH  - Teratocarcinoma/*metabolism/pathology
MH  - Tumor Cells, Cultured
MH  - Virulence Factors, Bordetella/pharmacology
MH  - Wnt Proteins
MH  - *Xenopus Proteins
MH  - *Zebrafish Proteins
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33539 [doi]
AID - S0021-9258(17)46497-0 [pii]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33539-44. doi: 10.1074/jbc.274.47.33539.