PMID- 10559237
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - Molecular cloning and characterization of human trabeculin-alpha, a giant protein
      defining a new family of actin-binding proteins.
PG  - 33522-30
AB  - We describe the molecular cloning and characterization of a novel giant human
      cytoplasmic protein, trabeculin-alpha (M(r) = 614,000). Analysis of the deduced
      amino acid sequence reveals homologies with several putative functional domains, 
      including a pair of alpha-actinin-like actin binding domains; regions of homology
      to plakins at either end of the giant polypeptide; 29 copies of a spectrin-like
      motif in the central region of the protein; two potential Ca(2+)-binding EF-hand 
      motifs; and a Ser-rich region containing a repeated GSRX motif. With similarities
      to both plakins and spectrins, trabeculin-alpha appears to have evolved as a
      hybrid of these two families of proteins. The functionality of the actin binding 
      domains located near the N terminus was confirmed with an F-actin binding assay
      using glutathione S-transferase fusion proteins comprising amino acids 9-486 of
      the deduced peptide. Northern and Western blotting and immunofluorescence studies
      suggest that trabeculin is ubiquitously expressed and is distributed throughout
      the cytoplasm, though the protein was found to be greatly up-regulated upon
      differentiation of myoblasts into myotubes. Finally, the presence of cDNAs
      similar to, yet distinct from, trabeculin-alpha in both human and mouse suggests 
      that trabeculins may form a new subfamily of giant actin-binding/cytoskeletal
      cross-linking proteins.
FAU - Sun, Y
AU  - Sun Y
AD  - Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts
      02115, USA.
FAU - Zhang, J
AU  - Zhang J
FAU - Kraeft, S K
AU  - Kraeft SK
FAU - Auclair, D
AU  - Auclair D
FAU - Chang, M S
AU  - Chang MS
FAU - Liu, Y
AU  - Liu Y
FAU - Sutherland, R
AU  - Sutherland R
FAU - Salgia, R
AU  - Salgia R
FAU - Griffin, J D
AU  - Griffin JD
FAU - Ferland, L H
AU  - Ferland LH
FAU - Chen, L B
AU  - Chen LB
LA  - eng
SI  - GENBANK/AF141968
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (MACF1 protein, human)
RN  - 0 (Macf1 protein, mouse)
RN  - 0 (Microfilament Proteins)
RN  - 22144-77-0 (Cytochalasin D)
RN  - SH1WY3R615 (Nocodazole)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Cytochalasin D/pharmacology
MH  - Humans
MH  - Microfilament Proteins/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Nocodazole/pharmacology
MH  - Sequence Homology, Amino Acid
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33522 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33522-30. doi: 10.1074/jbc.274.47.33522.