PMID- 10559234
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - ESkine, a novel beta-chemokine, is differentially spliced to produce secretable
      and nuclear targeted isoforms.
PG  - 33496-503
AB  - Using the murine embryonal stem cell system, we have identified a novel gene
      encoding a highly divergent member of the beta-chemokine family of
      proinflammatory mediators and have called this protein ESkine. Much of the coding
      sequence for ESkine overlaps with the 3'-end of a novel interleukin 11 receptor
      alpha-like sequence on murine chromosome 4. ESkine is produced as two splice
      variants. One of these variants encodes a classical chemokine with an associated 
      signal peptide, while the other variant (PESKY) possesses the main body of the
      chemokine but has replaced the signal peptide with an alternative stretch of
      amino acids that allows for nuclear targeting of this isoform. This differential 
      splicing arises as a result of alternative 5' exon usage. These differentially
      spliced forms are expressed at discrete tissue loci. Thus, while ESkine is highly
      expressed in the placenta, PESKY is mainly expressed in the Testes and brain and 
      weakly in the developing embryo. Studies on the proinflammatory properties of
      ESkine reveal it to be active in inducing polarization of CD4(+) T cells but to
      be inactive on other hemopoietic cellular populations.
FAU - Baird, J W
AU  - Baird JW
AD  - Beatson Institute for Cancer Research, Cancer Research Campaign Beatson
      Laboratories, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, United
      Kingdom.
FAU - Nibbs, R J
AU  - Nibbs RJ
FAU - Komai-Koma, M
AU  - Komai-Koma M
FAU - Connolly, J A
AU  - Connolly JA
FAU - Ottersbach, K
AU  - Ottersbach K
FAU - Clark-Lewis, I
AU  - Clark-Lewis I
FAU - Liew, F Y
AU  - Liew FY
FAU - Graham, G J
AU  - Graham GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCL27 protein, human)
RN  - 0 (Chemokine CCL27)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - CD4-Positive T-Lymphocytes/cytology
MH  - Cell Line
MH  - Cell Movement/physiology
MH  - Cell Nucleus/*metabolism
MH  - Chemokine CCL27
MH  - Chemokines/chemistry/*genetics/physiology
MH  - Chemokines, CC/chemistry/*genetics/physiology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Protein Isoforms/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Subcellular Fractions/metabolism
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33496 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33496-503. doi: 10.1074/jbc.274.47.33496.