PMID- 10559189
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190524
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - The homeodomain protein NK-3 recruits Groucho and a histone deacetylase complex
      to repress transcription.
PG  - 33194-7
AB  - Transcriptional repression by sequence-specific DNA binding factors is mediated
      by the recruitment of a corepressor complex to the promoter region. The NK-3
      homeodomain protein is a transcriptional repressor that recruits the nuclear
      protein kinase, homeodomain interacting protein kinase 2 (HIPK2). Here we show
      that HIPK2 is a component of a corepressor complex containing Groucho and a
      histone deacetylase complex. Groucho, like HIPK2, acts as a corepressor for NK-3 
      and binds to NK-3 and HIPK2. Moreover, HIPK2 appears to regulate the corepressor 
      activity of Groucho. Transcriptional repression by NK-3 and Groucho is relieved
      by the histone deacetylase inhibitor trichostatin A, and both NK-3 and Groucho
      directly interact with the histone deacetylase HDAC1 that is associated with
      mSin3A in vivo. Recruitment of the histone deacetylase complex by NK-3 decreases 
      the acetylated histones that are associated with the target gene promoter. These 
      results indicate that NK-3 represses transcription by recruiting a complex
      containing Groucho and a histone deacetylase complex that leads to histone
      modification on chromatin and suggest that HIPK2 may play a regulatory role in
      the corepressor complex formation.
FAU - Choi, C Y
AU  - Choi CY
AD  - Laboratory of Molecular Cardiology, NHLBI, National Institutes of Health,
      Bethesda, Maryland 20892, USA.
FAU - Kim, Y H
AU  - Kim YH
FAU - Kwon, H J
AU  - Kwon HJ
FAU - Kim, Y
AU  - Kim Y
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (gro protein, Drosophila)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.- (HIPK2 protein, human)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Carrier Proteins/metabolism
MH  - DNA Primers
MH  - DNA-Binding Proteins/*metabolism
MH  - HeLa Cells
MH  - Histone Deacetylases/*metabolism
MH  - Homeodomain Proteins/*metabolism
MH  - Humans
MH  - Protein Kinases/metabolism
MH  - *Protein-Serine-Threonine Kinases
MH  - Repressor Proteins/*metabolism
MH  - *Transcription, Genetic
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33194 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33194-7. doi: 10.1074/jbc.274.47.33194.