PMID- 10559186
OWN - NLM
STAT- MEDLINE
DCOM- 19991214
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 47
DP  - 1999 Nov 19
TI  - The gamma subunit modulates Na(+) and K(+) affinity of the renal Na,K-ATPase.
PG  - 33183-5
AB  - The Na(+),K(+)-ATPase catalyzes the active transport of ions. It has two
      necessary subunits, alpha and beta, but in kidney it is also associated with a
      7.4-kDa protein, the gamma subunit. Stable transfection was used to determine the
      effect of gamma on Na, K-ATPase properties. When isolated from either kidney or
      transfected cells, alphabetagamma had lower affinities for both Na(+) and K(+)
      than alphabeta. A post-translational modification of gamma selectively eliminated
      the effect on Na(+) affinity, suggesting three configurations (alphabeta,
      alphabetagamma, and alphabetagamma*) conferring different stable properties to
      Na, K-ATPase. In the nephron, segment-specific differences in Na(+) affinity have
      been reported that cannot be explained by the known alpha and beta subunit
      isoforms of Na,K-ATPase. Immunofluorescence was used to detect gamma in rat renal
      cortex. Cortical ascending limb and some cortical collecting tubules lacked
      gamma, correlating with higher Na(+) affinities in those segments reported in the
      literature. Selective expression in different segments of the nephron is
      consistent with a modulatory role for the gamma subunit in renal physiology.
FAU - Arystarkhova, E
AU  - Arystarkhova E
AD  - Laboratory of Membrane Biology, Massachusetts General Hospital, Charlestown,
      Massachusetts 02129, USA.
FAU - Wetzel, R K
AU  - Wetzel RK
FAU - Asinovski, N K
AU  - Asinovski NK
FAU - Sweadner, K J
AU  - Sweadner KJ
LA  - eng
GR  - HL36271/HL/NHLBI NIH HHS/United States
GR  - NS27653/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 9NEZ333N27 (Sodium)
RN  - EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - DNA Primers
MH  - Dogs
MH  - Kidney/*enzymology
MH  - Potassium/*metabolism
MH  - Rats
MH  - Sodium/*metabolism
MH  - Sodium-Potassium-Exchanging ATPase/chemistry/genetics/*metabolism
MH  - Swine
MH  - Transfection
EDAT- 1999/11/24 00:00
MHDA- 1999/11/24 00:01
CRDT- 1999/11/24 00:00
PHST- 1999/11/24 00:00 [pubmed]
PHST- 1999/11/24 00:01 [medline]
PHST- 1999/11/24 00:00 [entrez]
AID - 10.1074/jbc.274.47.33183 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Nov 19;274(47):33183-5. doi: 10.1074/jbc.274.47.33183.