PMID- 10558993
OWN - NLM
STAT- MEDLINE
DCOM- 19991130
LR  - 20190619
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 286
IP  - 5443
DP  - 1999 Nov 12
TI  - Activation of PPARgamma coactivator-1 through transcription factor docking.
PG  - 1368-71
AB  - Transcriptional coactivators have been viewed as constitutively active
      components, using transcription factors mainly to localize their functions. Here,
      it is shown that PPARgamma coactivator-1 (PGC-1) promotes transcription through
      the assembly of a complex that includes the histone acetyltransferases steroid
      receptor coactivator-1 (SRC-1) and CREB binding protein (CBP)/p300. PGC-1 has a
      low inherent transcriptional activity when it is not bound to a transcription
      factor. The docking of PGC-1 to peroxisome proliferator-activated receptor gamma 
      (PPARgamma) stimulates an apparent conformational change in PGC-1 that permits
      binding of SRC-1 and CBP/p300, resulting in a large increase in transcriptional
      activity. Thus, transcription factor docking switches on the activity of a
      coactivator protein.
FAU - Puigserver, P
AU  - Puigserver P
AD  - Dana-Farber Cancer Institute and Department of Cell Biology, Harvard Medical
      School, Boston, MA 02115, USA.
FAU - Adelmant, G
AU  - Adelmant G
FAU - Wu, Z
AU  - Wu Z
FAU - Fan, M
AU  - Fan M
FAU - Xu, J
AU  - Xu J
FAU - O'Malley, B
AU  - O'Malley B
FAU - Spiegelman, B M
AU  - Spiegelman BM
LA  - eng
GR  - DK54477/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nuclear Respiratory Factors)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (peroxisome-proliferator-activated receptor-gamma coactivator-1)
RN  - EC 2.3.1.48 (E1A-Associated p300 Protein)
RN  - EC 2.3.1.48 (Ep300 protein, mouse)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Ncoa1 protein, mouse)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - COS Cells
MH  - DNA-Binding Proteins/metabolism
MH  - E1A-Associated p300 Protein
MH  - Gene Expression Regulation
MH  - Histone Acetyltransferases
MH  - Mice
MH  - Nuclear Proteins/chemistry/*metabolism
MH  - Nuclear Receptor Coactivator 1
MH  - Nuclear Respiratory Factors
MH  - Protein Binding
MH  - Protein Conformation
MH  - Receptors, Cytoplasmic and Nuclear/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Trans-Activators/chemistry/*metabolism
MH  - Transcription Factors/chemistry/*metabolism
MH  - *Transcription, Genetic
MH  - Transfection
EDAT- 1999/11/13 00:00
MHDA- 1999/11/13 00:01
CRDT- 1999/11/13 00:00
PHST- 1999/11/13 00:00 [pubmed]
PHST- 1999/11/13 00:01 [medline]
PHST- 1999/11/13 00:00 [entrez]
AID - 7987 [pii]
AID - 10.1126/science.286.5443.1368 [doi]
PST - ppublish
SO  - Science. 1999 Nov 12;286(5443):1368-71. doi: 10.1126/science.286.5443.1368.