PMID- 10557092
OWN - NLM
STAT- MEDLINE
DCOM- 19991203
LR  - 20171116
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 44
DP  - 1999 Oct 28
TI  - The physical association and phosphorylation of Cdc25C protein phosphatase by
      Prk.
PG  - 6029-36
AB  - prk encodes a protein serine/threonine kinase involved in regulating M phase
      functions during the cell cycle. We have expressed His6-Prk and His6-Cdc25C
      proteins using the baculoviral vector expression system. Purified recombinant
      His6-Prk, but not a kinase-defective mutant His6-PrkK52R, is capable of strongly 
      phosphorylating His6-Cdc25C in vitro. Co-immunoprecipitation and affinity column 
      chromatography experiments demonstrate that GST-Prk and native Cdc25C interact.
      When co-infected with His6-Prk and His6-Cdc25C recombinant baculoviruses, sf-9
      cells produce His6-Cdc25C antigen with an additional slower mobility band on
      denaturing polyacrylamide gels compared with cells infected with His6-Cdc25C
      baculovirus alone. In addition, His6-Cdc25C immunoprecipitated from sf-9 cells
      co-infected with His6-Prk and His6-Cdc25C baculoviruses, but not with
      His6-PrkK52R and His6-Cdc25C baculoviruses, contains a greatly enhanced kinase
      activity that phosphorylates His6-Cdc25C in vitro. Moreover, phosphopeptide
      mapping shows that His6-Prk phosphorylates His6-Cdc25C at two sites in vitro and 
      that the major phosphorylation site co-migrates with the one that is
      phosphorylated in vivo in asynchonized cells. Further studies reveal that
      His6-Prk phosphorylates Cdc25C on serine216, a residue also phosphorylated by
      Chk1 and Chk2. Together, these observations strongly suggest that Prk's role in
      mitosis is at least partly mediated through direct regulation of Cdc25C.
FAU - Ouyang, B
AU  - Ouyang B
AD  - Division of Hematology/Oncology, Department of Internal Medicine, University of
      Cincinnati College of Medicine; ML-508, K-pavilion, 231 Bethesda Avenue,
      Cincinnati, Ohio, OH 45267-0508, USA.
FAU - Li, W
AU  - Li W
FAU - Pan, H
AU  - Pan H
FAU - Meadows, J
AU  - Meadows J
FAU - Hoffmann, I
AU  - Hoffmann I
FAU - Dai, W
AU  - Dai W
LA  - eng
GR  - R01-74229/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - EC 2.7.1.- (protein kinase N)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.3.48 (CDC25C protein, human)
RN  - EC 3.1.3.48 (cdc25 Phosphatases)
SB  - IM
MH  - Animals
MH  - Baculoviridae/genetics
MH  - Blotting, Western
MH  - Cell Cycle/genetics
MH  - Cell Cycle Proteins/genetics/isolation & purification/*metabolism
MH  - Cells, Cultured
MH  - Glutathione Transferase/genetics/metabolism
MH  - Humans
MH  - Insecta/cytology/virology
MH  - Phosphorylation
MH  - Precipitin Tests
MH  - Protein Kinase C
MH  - Protein-Serine-Threonine Kinases/genetics/isolation & purification/*metabolism
MH  - Recombinant Proteins/genetics/isolation & purification/metabolism
MH  - cdc25 Phosphatases/genetics/isolation & purification/*metabolism
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - 10.1038/sj.onc.1202983 [doi]
PST - ppublish
SO  - Oncogene. 1999 Oct 28;18(44):6029-36. doi: 10.1038/sj.onc.1202983.