PMID- 10557082
OWN - NLM
STAT- MEDLINE
DCOM- 19991210
LR  - 20091119
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 43
DP  - 1999 Oct 21
TI  - Functional interaction of vascular endothelial-protein-tyrosine phosphatase with 
      the angiopoietin receptor Tie-2.
PG  - 5948-53
AB  - During development of the vertebrate vascular system essential signals are
      transduced via protein-tyrosine phosphorylation. Null-mutations of
      receptor-tyrosine kinase (RTK) genes expressed in endothelial cells (ECs) display
      early lethal vascular phenotypes. We aimed to identify endothelial
      protein-tyrosine phosphatases (PTPs), which should have similar importance in
      EC-biology. A murine receptor-type PTP was identified by a degenerated PCR
      cloning approach from endothelial cells (VE-PTP). By in situ hybridization this
      phosphatase was found to be specifically expressed in vascular ECs throughout
      mouse development. In experiments using GST-fusion proteins, as well as in
      transient transfections, trapping mutants of VE-PTP co-precipitated with the
      Angiopoietin receptor Tie-2, but not with the Vascular Endothelial Growth Factor 
      receptor 2 (VEGFR-2/Flk-1). In addition, VE-PTP dephosphorylates Tie-2 but not
      VEGFR-2. We conclude that VE-PTP is a Tie-2 specific phosphatase expressed in
      ECs, and VE-PTP phosphatase activity serves to specifically modulate
      Angiopoietin/Tie-2 function. Based on its potential role as a regulator of blood 
      vessel morphogenesis and maintainance, VE-PTP is a candidate gene for inherited
      vascular malformations similar to the Tie-2 gene.
FAU - Fachinger, G
AU  - Fachinger G
AD  - Max-Planck-Institute for Physiological and Clinical Research, W.G. Kerckhoff
      Institute, Department of Molecular Cell Biology, Parkstrasse 1, D-61231 Bad
      Nauheim, Germany.
FAU - Deutsch, U
AU  - Deutsch U
FAU - Risau, W
AU  - Risau W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, TIE-2)
RN  - EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor)
RN  - EC 3.1.3.48 (PTPRZ1 protein, human)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
RN  - EC 3.1.3.48 (Ptprz1 protein, mouse)
RN  - EC 3.1.3.48 (Receptor-Like Protein Tyrosine Phosphatases, Class 5)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - Endothelium, Vascular/*enzymology
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Phosphorylation
MH  - Protein Tyrosine Phosphatases/genetics/*metabolism
MH  - Receptor Protein-Tyrosine Kinases/genetics/*metabolism
MH  - Receptor, TIE-2
MH  - Receptor-Like Protein Tyrosine Phosphatases, Class 5
MH  - Receptors, Growth Factor/genetics/metabolism
MH  - Receptors, Vascular Endothelial Growth Factor
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - 10.1038/sj.onc.1202992 [doi]
PST - ppublish
SO  - Oncogene. 1999 Oct 21;18(43):5948-53. doi: 10.1038/sj.onc.1202992.