PMID- 10557056
OWN - NLM
STAT- MEDLINE
DCOM- 19991207
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 13
IP  - 11
DP  - 1999 Nov
TI  - Detection of MUM1/IRF4-IgH fusion in multiple myeloma.
PG  - 1812-6
AB  - MUM1 (multiple myeloma oncogene 1)/IRF4 (interferon regulatory factor 4) gene has
      been identified as an oncogene transcriptionally activated by t(6;14)(p25;q32)
      chromosomal translocation in multiple myeloma (MM). The significance of this
      alteration in MM remains unknown, as it is not detectable by means of
      conventional cytogenetic analysis. To address this issue, we established
      diagnostic procedures based on pulsed-field gel electrophoresis (PFGE) analysis
      and double color fluorescence in situ hybridization (DCFISH) using DNA probes
      derived from the MUM1 and the immunoglobulin heavy chain (IgH) gene loci. Among a
      panel of 17 MM cell lines, three (17.6%) showed fusions between these two loci,
      which resulted in the juxtaposition of the MUM1 to the IgH 3' alpha-enhancer
      region by virtue of t(6;14) or insertion of the IgH sequences into the vicinity
      of the MUM1 gene and in the concomitant overexpression of the MUM1 mRNA. With
      similar results, fusions between MUM1 and IgH loci were observed by means of
      interphase DCFISH in eight (21.1%) out of the 38 MM cases, although no definite
      relationships between MUM1 status and specific clinical findings could be
      established.
FAU - Yoshida, S
AU  - Yoshida S
AD  - Second Department of Internal Medicine, Nagoya City University Medical School,
      Nagoya, Japan.
FAU - Nakazawa, N
AU  - Nakazawa N
FAU - Iida, S
AU  - Iida S
FAU - Hayami, Y
AU  - Hayami Y
FAU - Sato, S
AU  - Sato S
FAU - Wakita, A
AU  - Wakita A
FAU - Shimizu, S
AU  - Shimizu S
FAU - Taniwaki, M
AU  - Taniwaki M
FAU - Ueda, R
AU  - Ueda R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GCGGCCGC-specific type II deoxyribonucleases)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Chromosomes, Human, Pair 6/genetics
MH  - DNA Mutational Analysis
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Enhancer Elements, Genetic/genetics
MH  - Female
MH  - Genes, Immunoglobulin/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/diagnosis/*genetics
MH  - Oncogene Proteins, Fusion/*genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1999/11/11 00:00
MHDA- 1999/11/11 00:01
CRDT- 1999/11/11 00:00
PHST- 1999/11/11 00:00 [pubmed]
PHST- 1999/11/11 00:01 [medline]
PHST- 1999/11/11 00:00 [entrez]
AID - 10.1038/sj.leu.2401563 [doi]
PST - ppublish
SO  - Leukemia. 1999 Nov;13(11):1812-6. doi: 10.1038/sj.leu.2401563.